摘要
目的探讨多参数流式细胞术(FCM)在白血病免疫分型中的应用。方法利用CD45/SSC设门的四色荧光FCM检测339例白血病患者骨髓细胞表面标记。结果 339例白血病中急性髓系白血病(AML)150例,占44.2%,髓系抗原表达率以CD33(86.7%)最高,其余从高到低依次为CD117、CD3、cMPO、CD15。淋巴细胞性白血病183例,占54.0%,其中急性B淋巴细胞白血病(ALL-B)110例、急性T淋巴细胞白血病(ALL-T)27例、慢性淋巴细胞白血病(CLL)46例。ALL-B淋巴系抗原表达率以CD19最高,其余由高到低依次为cCD79a、CD10、CD22、CD20,ALL-T淋巴系抗原表达率以CD7最高,其余由高到低依次为CD2、CD5、CD3。髓系白血病中常见交叉表达淋巴系抗原CD56、CD7、CD9。ALL中常见交叉表达髓系抗原CD13、CD33,CD14的表达与单核细胞白血病相关。结论应用多参数FCM能对白血病进行分型,对于白血病的诊断治疗和预后判断均有重要价值。
Objective To observe the application and clinical significance of multi-parameter flow cytometry（FCM） for the immunophenotyping of leukemia.Methods Immunophenotyping of 339 cases of bone marrow specimens from patients with leukemia were analyzed by four color FCM with the method of CD45/SSC double parameter and scatter spot picture.Results In the 339 cases of leukemia,acute myeloid leukemia（AML） was for 150 cases（44.2%）,and among medullary system antigens,CD33 expression rate was the highest（86.7%）,followed by CD117,CD3,cMPO and CD15.Lymphocytic leukemia was for 183 cases（54.0%）,including acute lymphoblastic leukemia（ALL）-B 110 cases,ALL-T 27 cases and chronic lymphoblastic leukemia（CLL） 46 cases.Among B-series of lymphocyte system antigens,expression rate of CD19 was the highest,followed by cCD79a,CD10,CD22 and CD20.Among T-series of lymphocyte system antigens,expression rate of CD7 was the highest,followed by CD2,CD5 and CD3.CD56,CD7 and CD9 were the common cross-expressed lymphocyte system antigens in myeloid leukemia,CD13 and CD3 were the common cross-expressed myeloid system antigens in ALL,and the expression of CD14 might be associated with monocytic leukemia.Conclusion Multi-parameter FCM could be used for the identification of leukemia,and be with great value for the diagnosis,therapy and prognosis of leukemia.
出处
《国际检验医学杂志》
CAS
2012年第12期1427-1429,共3页
International Journal of Laboratory Medicine
关键词
白血病
流式细胞术
免疫分型
leukemia； flow cytometry； immunophenotyping
