摘要
本研究就人工培养冬虫夏草菌粉对小鼠细胞的免疫抑制作用进行了体内外观察。虫草菌粉0.6～5mg/ml 均能显著抑制小鼠外周血白细胞吞噬功能、脾淋巴细胞增殖反应、混合淋巴细胞培养以及 LPS 诱导的巨噬细胞 IL-1生成(P<0.05～0.01)。其抑制强度与虫草菌粉剂量成正比例。5mg/ml 虫草菌粉可完全抑制上述细胞免疫反应,而该剂量药物与脾细胞共育5天也不影响细胞的存活率。给同种异体皮肤移植小鼠每日口服虫草菌粉4g/kg,使皮片存活时间(12.7±2.2天)较对照组(8.3±0.7天)显著延长(P<0.01),近似于环抱霉素 A(每日5mg/kg 口服)抑制皮片排异的效果(13±2.9天,P>0.7)。
The immunosuppressive effect of cultured Cordyceps sinensis(Bei Lin Capsule)was studied in vitro and in vivo.When the drug was added from 0.6 mg/ml to 5 mg/ml a significant dose-dependent inhibition effect was shown in the following immune reactions of mice(P<0.05～0. 01):phagocytic function of peripheral bh)od leucocytes assayed by chemiluminescence;mitogenic response of spleen lymphocytes to Con A;mixed lymphocyte culture and LPS induced interleukin-1 release of macrophages.The survival rate of mice spleen lymphocytes cultured with Cordyceps sinensis 5 mg/ml in 37℃ 5% CO_2 for 5 days was more than 80%.Cordyceps sinensis 4 g/kg daily significantly prolonged the mice skin allograft survival time(12.7±2.2 days v.s 8.3±0.7 days in the control,P<0.01)and its immunosuppressive effect was close to that of Cyclosporin A 5 mg/kg daily on skin allograft.
