摘要
目的:建立麝香酮在大鼠肠灌注液中的GC-MS/MS测定方法,研究麝香酮的大鼠小肠吸收动力学特征。方法:采用GC-MS/MS测定大鼠肠循环液中的麝香酮含量;采用大鼠原位肠循环灌注法进行麝香酮吸收动力学研究。结果:麝香酮在各小肠段均有较好吸收;其吸收速度常数(Ka)和每小时吸收率(A),十二指肠>空肠(P<0.05),十二指肠>回肠(P<0.01)。麝香酮在小肠段的Ka,A和吸收半衰期T1/2分别为0.990 h-1,43.58%,0.705 h。结论:麝香酮在整个肠道内均有较好吸收,十二指肠Ka,T1/2优于空肠(P<0.05),十二指肠Ka,T1/2,A显著优于回肠(P<0.01,P<0.01,P<0.05),空肠与回肠无显著差异。
Objective： To establish the method for determining muscone in rat intestinal perfusate by GC-MS/MS and study its intestinal absorption kinetic characteristics in rats. Method ： The GC-MS/MS method was used to determine the content of muscone in rat intestinal circulation fluid. In situ intestinal circulation peffusion was adopted to study absorption kinetics of muscone in rats. Result ： Muscone was proved to be well absorbed in each section of small intestine. Its absorption rate constants （ Ka ） and the absorption rate （A） in the rat intestine showed duodenum 〉jejunum （P 〈0, 05）, duodenum 〉 ileum （P 〈0. 01 ）. Its Ka, A and t1/2 in rat small intestine was 0. 990 h -1, 43.58% and 0. 705 h, respectively. Conclusion： Muscone was well absorbed in each intestinal section, with duodenum better than jejunum （Ka, T1/2, P 〈 0. 05） significantly better than ileum （Ka, T,/2, P 〈 0. 01 ; A, P 〈 0. 05）. There is no obvious statistical difference between jejunum and ileum.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第16期2456-2460,共5页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81001650)
关键词
麝香酮
吸收动力学
原位肠灌注
GC-MS/MS
muscone
absorption kinetics
in situ intestinal circulation peffusion
GC-MS/MS
