摘要
目的采用稳定转染pcDNA3.1(+)/mBD2的细胞株复制裸鼠移植瘤模型,通过对荷瘤小鼠肿瘤生长、瘤组织凋亡等指标的观察,揭示mBD2在动物体内抗肿瘤作用机制。方法将稳定转染PcDNA3.1(+)/mBD2、PcDNA3.1(+)的细胞株SiHa/M、SiHa/K及正常SiHa细胞于裸鼠左前腋下皮下接种,复制裸鼠移植瘤模型。观察肿瘤生长状况、荷瘤小鼠存活时间及肿瘤组织病理改变,采用免疫组织化学方法测定肿瘤组织中目的蛋白的表达,并用TUNEL法检测肿瘤组织的细胞凋亡。结果成功复制了裸鼠宫颈癌移植瘤模型。肿瘤生长曲线显示,SiHa/M组裸鼠肿瘤生长慢于SiHa/K组和SiHa组(P<0.05);SiHa/M肿瘤直径小于SiHa组和SiHa/K组,差异均有统计学意义(P<0.05)。肿瘤组织HE染色结果显示,SiHa/M细胞生长不活跃,局部有出血坏死,病理性核分裂相少;SiHa/K组与SiHa组肿瘤细胞生长活跃,病理性核分裂相多见,瘤细胞侵犯周围脂肪及肌肉组织。免疫组织化学染色结果显示SiHa/M组有mBD2表达;由TUNEL染色结果可以看出,SiHa/M组细胞凋亡较多,而SiHa/K及SiHa组细胞凋亡少,两者之间的差异有统计学意义(P<0.05)。结论 mBD2能够抑制移植瘤的生长,其机制可能与mBD2诱导肿瘤细胞凋亡有关。
[Objective] The models of naive mice transplanting tumor were established with stably transfected pcDNA 3.1 （＋） /mBD2 for discovering the antitumor mechanism of mBD2 protein. [Methods] The models of naive mice transplanting tumor were established with subcutaneous injection cell lines of SiHa/M, SiHa/K transfected PcDNA 3.1 （＋） /mBD2 and PeDNA 3.1 （＋） and SiHa. The diameters of tumor were mea- sured and tumor growth curves were draw. mBD2 protein expression and pathological changes in tumor tissues were observed with HE and immuno-histoehemical staining. Apoptosis in the transplanted tumor was detected by TUNEL method. [Results ] The models of naive mice transplanting tumor were established successful. The curve of tumor diameter showed growth velocity of SiHa and SiHa/K was faster than Siha/M （P 〈0.05）; the diameters of tumor of Siha/M were also smaller than SiHa and SiHa/K （P 〈0.05）; the pathological changes showed tumor growth of SiHa and SiHa/K was more active than SiHa/M, at the same time, apoptosis in SiHa and SiHa/K was less than SiHa/M （P〈0.05）. [Conclusions] mBD2 protein can restrain tumor growth by induced tumor apoptosis.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2012年第20期28-32,共5页
China Journal of Modern Medicine
关键词
裸鼠
移植瘤
TUNEL
凋亡
naive mice
transplanting tumor
TUNEL
apoptosis
