摘要
本实验使用组织培养技术对472种中草药(均含生药10mg/ml)进行了抗Ⅰ型单纯疱疹病毒的初筛(定性)和复筛(定量)研究。水与乙醇提取物初筛出有效药物,经复筛后有效率减少28.8～80.0%。4种给药途径的有效机率(以同时给药途径为基数)为管外>同时>治疗>预防。给药途径越多,一种药物的有效程度却越少。两种提取物或各给药途径经复筛出10种高效药物:青木香、刘寄奴、乌药、败普草、马尾松、夏枯草、石韦、盐肤木、海藻和蒲公英。
Using tissue culture method,the present work with its first-hand observation was primarily concerned with evaluating the antiviral effect of 472 traditional medicinal herbs (comprising raw material 10 mg/ml),through both initial (qualitative) and repeated (quantitative) screens,on type 1 herpes simplex virus.When dealing with water and alcoholic extracts,the effective herbs during initial screens were reduced after repeated screens by a range of 28.8～80.0%.Employing the basic value attained by the simultaneous route of drug administration,a stepwise declining of effective herbs would be:extratube route>simultaneous route>therapeutic route>preventive route.The more the routes of drug administration,the less the multiple-route simultaneous efficacy of a herb. Through repeated screens,10 highly effective herbs were Aristolochia debilis,Artemisia anomala, Lindera strychnifolia,Patrinia villosa,Pinus massoniana,Prunella vulgaris,Pyrrosia lingua,Rhus chinensis,Sargussum fusiforme and Taraxacum mongolicum,Clinically,among the 78 cases of herpetic keratitis due to HSV,treated by Pyrrosia lingua and Prunella vulgaris eye drops,a cure was effected in 38 amd an improvement in 37,with 3 being of no benefit.
基金
国家自然科学基金 No 3860721
