摘要
光敏色素是一类与作物农艺性状密切相关的基因家族。本研究克隆了中国春小麦光敏色素TaPHYA1、TaPHYA2和TaPHYA3基因完整的编码序列,并对它们进行了生物信息学以及转录分析。在NCBI数据库中对其推测的氨基酸序列进行BLAST分析,发现TaPHYA1和TaPHYA3氨基酸序列中包含光敏色素基因完整的功能结构域。系统进化树分析表明,小麦TaPHYA1、TaPHYA2和TaPHYA3之间进化关系相近,且与单子叶植物玉米、水稻的PHYA聚为一个亚类。另外,TaPHYA的表达丰度具有组织特异性,在茎、叶、穗中表达量分别是根中的1.35、0.34和0.87倍;而在同光质条件下,其表达丰度也具有光质特异性,TaPHYA的总表达量在黑暗和远红光条件下最高,在蓝光条件下次之,而红光和白光条件下最低。该结果为深入研究小麦光敏色素A基因亚家族的功能奠定了基础。
Phytochromes（PHYs） are important genes related to crop agronomic traits.In this paper,TaPHYA1,TaPHYA2,and TaPHYA3 were cloned from cv.Chinese Spring（Triticum aestivum L.）.The putative domains of TaPHYA1,TaPHYA2,and TaPHYA3 proteins were predicted via the NCBI Protein BLAST.Either TaPHYA1 or TaPHYA3 is composed of a GAF domain,a PHYtochrome domain,two PAS domains,a His Kinase A domain,and a Histidine kinase-like ATPase domain.Phylogenetic tree analysis indicated that TaPHYA1,TaPHYA2,and TaPHYA3 are more close to PHYA members of monocot plants（ZmPHYA,SbPHYA,and OsPHYA） rather than to those of dicot plants（AtPHYA and GmPHYA）.Expression profile of TaPHYAs were analyzed using semi quantitative RT-PCR and real time PCR assays.We found that the expression levels of TaPHYAs in stem,leaf and spike were 1.35,0.34,and 0.87 times of that in root,respectively.Additionally,TaPHYA showed high expression level in darkness,far-red,and blue light,but low expression level in red and white light conditions.The transcription of TaPHYA in the seedlings grown in the dark or far-red light was four or three times of that in seedlings grown in red light,respectively.
出处
《作物学报》
CAS
CSCD
北大核心
2012年第8期1354-1360,共7页
Acta Agronomica Sinica
基金
国家转基因生物新品种培育重大专项(2011ZX08010-002)
国家重点基础研究发展计划(973计划)项目(2009CB118300)
国家高技术研究发展计划(863计划)项目(2008AA10Z121)资助
关键词
小麦
光敏色素A
组织特异性
表达分析
Triticum aestivum； Phytochrome A； Tissue-specific expression； Transcription expression analysis
