摘要
用HBVDNA转染体外细胞系 (人肝肿瘤细胞株 )而得到一种瞬间表达产物通过检验这种产物来研究X基因的表达特性 ,并通过同时转染抑癌基因P53 来观察其对X基因在人肝细胞中表达的抑制作用。结果提示 :P53 可通过抑制X检验在细胞中的表达而使其转活性功能丧失从而达到抑制癌肿产生的作用 ,但P53 抑癌机能的发挥与其在细胞中含量的多少有关。
Purpose Hepatitis B Virus(HBV) is a major risk factor for human hepatocellular carcinoma. The oncogenicity of HBV is dependent on the intigration of the HBV X gene into the hepatocellular DNA and transactive function to the cellular gene.The cellular turmor-suppressor gene p 53 can negatively regulate the transactive function of X gene. To examine the expression of the X gene in hepatoma cells and the inhibiting function of P 53 gene to the X gene. This test was conducted.Methods The methods were analysing the production of transient expression of transfected HBV DNA, HBV X gene and P 53 using human hepatocellular carcinoma HuH-7 and HeG 2 cells, as a recipient by cell culture in vitro and southern hybridization.Results The 2.6,2.2 and 0.9 kilobase transcripts were observed. We also found that wild-type p 53 markedly reduced the expreseion of HBV S gene and X gene as complexed they to transfect the hepatocellular carcinoma cells.Conclustins Results suggest that wild-type p 53 inhibit the transactive function of HBV X gene in the liver cells.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2000年第7期593-595,共3页
Chinese Journal of Public Health
基金
辽宁省科委重大科技项目
