摘要
目的:观察Jagged1RNA干扰慢病毒载体抑制舌癌Cal-27细胞增殖、侵袭作用。方法:采用RT-PCR和Western Blot检测Jagged1siRNA对Cal-27细胞Jagged1基因和蛋白表达水平的敲减效果;克隆形成实验检测干扰后细胞群体依赖性和增殖能力;流式细胞仪检测细胞周期的变化;Tranwell检测RNA干扰后侵袭率变化。结果:KD组较NC组Jagged1基因和蛋白表达受到明显的敲减。KD组细胞克隆形成率明显降低,较NC组下降42.42%。S期细胞比率均明显下调,约62.98%,G1期细胞比率上调,凋亡率明显增高。72hKD细胞生长抑制率为32.50%。Jagged1RNAi慢病毒载体感染后,KD组侵袭率无下降。结论:下调Jagged1基因和蛋白的表达,可使舌癌细胞克隆形成率明显降低、增殖活性降低、S期细胞比率下降、凋亡率升高、成瘤能力下降。
Objective To investigate the role of lentivirus-mediated Jagged1 RNA interference in inhibiting the proliferation and invasion of tongue cancer cell Cal-27. Methods RT-PCR and Western blot assay was used to determine the knock-down efficiency of Jaggedl by RNA interference in Cal-27 cells. Clone formation experiment was performed to detect cell colony dependence and reproductive activity. Cell cycle was determined by flow cytometry, and cell invasion activity was determined by the chamber cell invasion assay. Results The siRNA of KD group had significant knock-down effect on Jaggedl. Colony formation of Cal-27 ceils was significantly reduced in the KD group. The percentage of S-phase in Cal-27 ceils was significantly reduced in the KD group after transfection with the lentiviral vectors. The percentage of S-phase in Cal-27 ceils in the KD group decreased by 62.98% compared to that in the NC group. The percentage of Gl-phase in Cal-27 cells in the KD group increased. Apoptosis rate of Cal-27 cells was significantly increased in the KD group. The cell growth in the KD group was inhibited at the rate of 32.50%. The cell invasion rate was not changed obviously after transfection with Jaggedl- targeted RNAi lentiviral vector. Conclusion Targeting Jaggedl RNA interference can efficiently decrease Jaggedl mRNA and protein expressionin Cal-27 cells, resulting in inhibition of tongue cancer cell proliferation s. Jaggedl is a promising new target for the treatment of tongue cancer.
出处
《实用医学杂志》
CAS
北大核心
2012年第16期2678-2681,共4页
The Journal of Practical Medicine
基金
国家自然科学基金(编号:30772442)
第5批中国博士后科学基金面上项目(编号:2012M511877)
