摘要
目的探讨手工制备浓缩血小板的影响因素,提高手工浓缩血小板的质量和临床输注效果。方法随机选择献血前72小时内未服用过阿斯匹林类药物的献血者,外周血小板计数正常,采血顺畅,采用相同的分离方法(富含血小板血浆法)制备手工浓缩血小板,观察不同规格的全血,或不同的离心条件对收集血小板的影响。结果 400ml全血分别在2000、2100和2200rpm,离心5min血小板为(84.0±14)、(90.5±10.5)、(3.89±0.48)109/L,离心6min血小板为(86.3±11.5)、(121.5±17.7)、(85.0±16.5)109/L,经统计学处理2100rpm离心6min结果与其它组比较差异显著(P<0.01);其它各组相互比较差异不显著(P>0.5)。全血量分别为400、300、200ml时分离出的血小板为(1.46±0.28)、(3.53±0.66)、(4.86±0.71)109/L,400、300ml间比较差异不显著(P>0.5),200ml与400、300ml间比较差异显著(P<0.01)。结论采用相同的分离法,不同的离心条件、血液规格收集血小板均有影响,400ml的全血,转速为2100rpm/min,6min,22℃离心制备的浓缩血小板含量最高,收集效果最好。
Objective To analyze the influence factors of handmade blood platelets concentrate and improve the quality and the clinical injection effect of handmade platelets concentrate.Methods The blood donors who hadn’t taken aspirin medicine before donating blood in 72 hours were randomly enrolled in this study;peripheral blood platelets counts were normal;blood were collected smoothly;then platelets concentrate were reserved by same centrifuging method（platelet-rich plasma method） to discuss the influence of centrifugal conditions or whole blood specifications on collecting platelets.Results The 400ml whole blood under 2000rpp,2100rpm,2200rpm,centrifugalize 5min platelets（unit：109/L） 84.0±14、90.5±10.5、3.89±0.48,and 6min platelets（unit：109/L） 86.3±11.5、121.5±17.7、85.0±16.5.The results under 2100rpm and centrifugalized 6min have significant difference,compared with other groups;There is no obvious difference between other groups（P〉0.5） The difference between the platelets（1.46±0.28、3.53±0.66、4.86±0.71） prepared with 400,300,200ml whole blood respectively is not obvious,however the difference is significant compared 200ml with 400,300ml（P〈0.01）.Conclusion Under the same separation method,but different centrifugation conditions,type of blood,the platelet concentrate prepared with 400 ml of whole blood under the condition that the rotating speed is 2100 rpm,the centrifuging time is 6 min,and the centrifuging temperature is 22 degrees centigrade has the highest content and the best collecting effect.
出处
《西部医学》
2012年第8期1626-1628,共3页
Medical Journal of West China
关键词
浓缩血小板
影响因素
离心条件
血液规格
Platelets concentrate
Influence factor
Centrifuging condition
Blood specification
