摘要
通过白菜型油菜‘R-O-18’和菜薹‘L58’的基因组重测序数据与白菜基因组的参考序列(‘Chiifu-401-42’的基因组序列)的比对,在全基因组范围内检测到了18479个短插入缺失变异位点(≤5bp)。从中挑选了500个插入/缺失片段为4～5bp的InDel变异位点,将其设计成InDel分子标记并进行试验验证,结果有452个标记通过PCR扩增出单一条带,但仅有106个在‘R-O-18’和‘L58’间表现出多态性,346个没有多态性,48个在PCR中没有扩增。亲本间具有多态性的106个InDel标记可用来检测以‘R-O-18’和‘L58’为亲本构建的RILs基因型,并构建了一张包含99个标记的遗传连锁图谱。
Genome-wide Brassica campestris short Insertion/Deletion length polymorphisms （InDels） （ ~〈 5 bp） were detected by aligning whole genome re-sequencing data from two B. campestris accessions, ＇R-O-18＇ and ＇L58＇ , to the reference genome sequence of ＇Chiifu-401-42＇ . In total, we identified 18 479 InDel polymorphsms between ＇R-O-18＇ and ＇L58＇ A total of 500 InDel polymorphisms （4 - 5 bp in length） were converted to InDel markers and experimentally validated. Of the selected 500 InDel polymorphisms, 452 yielded a single PCR fragment, but only 106 showed polymorphism between ＇R-O-18＇ and ＇L58＇, 48 did not amplify a product, 346 showed no polymorphism. These 106 InDel markers were used to screen the genotype of RILs developed from the crossing of ＇R-O-18＇ and ＇L58＇ . A genetic linkage map contained 99 InDel markers was constructed.
出处
《园艺学报》
CAS
CSCD
北大核心
2012年第8期1491-1500,共10页
Acta Horticulturae Sinica
基金
中央级公益性科研院所基本科研业务费专项(1610032011011)
