摘要
目的:测定多倍体和二倍体金银花、叶和茎中绿原酸的含量,研究其提取物的抑菌作用。方法:高效液相色谱法(HPLC)和最小抑菌浓度(MIC)测定法。多倍体金银花、叶、茎中绿原酸含量分别为:7.76%、4.61%、0;二倍体为:5.97%、3.94%、0。多倍体金银花、叶提取物对大肠杆菌、藤黄八叠球菌、枯草芽孢杆菌的MIC分别为:250、500、500、500、250、500μg/g,二倍体为:500、1000、500、500、500、1000μg/g。结论:多倍体金银花、叶中绿原酸的含量比二倍体金银花、叶有显著性的提高,多倍体金银花提取物的抑菌作用强于二倍体金银花提取物。多倍体金银花比二倍体金银花具有更高的开发价值。
The content of chlorogenic acid （CHA） in flower, leaf and stem of polyploid and diploid Honeysuckle was determined, and bacteriostasis of extracts was studied. High performance liquid chromatography（HPLC） and mininal inhibitory concentration （MIC）. The content of CHA in Honeysuckle flower, leaf and stem is, 7.76%, 4.61% and 0 in polyploids, 5.97%, 3.94% and 0 in diploids, respectively. The MIC of extracts from Honeysuckle flower and leaf for Escherichia coli, Microccus luteus （Schroeter） Cohn and Bacillus subtilis is, 250 μg/g and 500 μg/g, 500μg/g and 500 lag/g, 250 μg/g and 500μg/ g in polyploids, 500 μg/g and 1000 μg/g, 500 μg/g and 500μg/g, 500 μg/g and 1000 μg/g in diploids, respectively. The content of CHA in polyploid Honeysuckle flower, leaf is significantly improved comparedwith diploid Honeysuckle. The bacteriostasis of extract of polyploid Honeysuckle is superior to diploid Honeysuckle. Polyploid has a higher development value than diploid Honeysuckle.
出处
《食品科技》
CAS
北大核心
2012年第8期225-227,231,共4页
Food Science and Technology
