摘要
目的研究应用MTT测定法评估对MTAD、17%EDTA和2.6%NaOCl不同时间点的pH值对人牙周膜成纤维细胞的细胞毒性的影响.方法将人类牙周膜成纤维细胞浸泡于冲洗液中,1、6和12 h后测定细胞活性,培养基的pH值及使用酶标仪测定每种培养基的光吸收值.结果 2.6%NaOCl的细胞毒性显著低于17%EDTA和MTAD,同时所有的冲洗液的细胞毒性先上升后下降,且在12 h细胞毒性最低,pH与细胞毒性均存在显著相关.结论冲洗液细胞毒性与pH值的变化趋势一致,pH值的变化可能会导致冲洗液细胞毒性的改变.
Objectives To assess the possible impact of pH on cytotoxic effects of MTAD, 17% EDTA, and 2.6% NaOCI on the human periodontal ligament fibroblast cells using MTF assay. Methods Human periodontal ligament fibroblast cells were exposed to the irrigants and their viability was assessed after l, 6, and 12 h. The pH of the medium was measured in each interval. Light absorption values were measured for each culture medium using Elisa Reader device. Results 2.6%NaOC1 had significantly less cytotoxicity than 17% EDTA and MTAD. Also irrigants cytotoxicity decreased at 1, 6, and 12 h, respectively. Conclusion It seems that variation of the pH results in variation in the cytotoxicity of solutions; i.e., it follows the pattern of the pH variation.
出处
《昆明医学院学报》
2012年第7期122-125,131,共5页
Journal of Kunming Medical College
