摘要
旨在研究E-钙粘蛋白抗体(ECCD-1)对小鼠早期胚胎体内外发育的影响。在体外培养小鼠8-细胞胚胎的培养液中,分别添加不同浓度的ECCD-1,通过胚胎形态观察、碱性磷酸酶染色、多能性因子免疫染色、体外贴壁培养、嵌合体制备以及体内移植试验检测ECCD-1对小鼠胚胎发育能力的影响。结果表明,ECCD-1抗体延迟胚胎致密化和囊胚腔形成,但内细胞团并未受到影响,胚胎碱性磷酸酶和SSEA-1、Oct-4、Sox2、Nanog 4种多能性因子的免疫染色都呈阳性,此外,ECCD-1抗体也不影响胚胎的体外贴壁生长、体内移植着床以及嵌合体的制备。这些数据表明,ECCD-1抗体延迟胚胎致密化但并不影响胚胎的体内外发育能力。
To investigate the effects of E-cadherin antibody（ECCD-1） on mouse embryo development,ECCD-1 antibody with different concentrations was added into KSOM media when mouse 8-cell embryos were cultured in vitro,and then the developmental potential of ECCD-1-treated embryos were detected by morphology,alkaline phosphatase activity,pluripotent marker（SSEA-1,Oct-4,Sox-2,Nanog） immunostaining,attachment culture in vitro,implantation in vivo,and chimera production.The result showed that ECCD-1 significantly delayed embryo compaction and blastocoele formation.ECCD-1-treated embryos had obvious inner cell mass,positive alkaline phosphatase and pluripotent markers,normal attachment growth in vitro and implantation in vivo.In addition,the chimera production was also improved when 8-cell embryos injected by embryonic stem cells were treated with ECCD-1.The results indicated that compaction delay by ECCD-1 antibody did not affect embryo development potential.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第8期1222-1229,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(30871790)
河北省自然科学基金(C2009000600)
