摘要
目的:本研究旨在探讨横纹肌肉瘤(rhabdomyosarcoma,RMS)组织中缺氧诱导因子-1α(hypoxiainducible factor-1alpha,HIF-1α)和血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达及临床意义。方法:采用免疫组织化学法检测70例RMS组织HIF-1α和VEGF的表达,分析其与RMS患者临床病理特征及预后的相关性。结果:RMS组织中HIF-1α阳性表达率为75.7%(53/70),VEGF阳性表达率为72.9%(51/70)。统计结果显示,HIF-1α和VEGF的表达呈正相关(r=0.554,P=0.000)。HIF-1α蛋白表达与RMS患者的性别(P=0.035)、肿瘤大小(P=0.016)、分期(P=0.008)有相关性,但与患者的年龄(P=0.555)、病理亚型(P=0.625)及淋巴结转移(P=0.750)无相关性;HIF-1α蛋白表达阴性组与表达阳性组患者的3年总生存率分别为54.5%及38.0%(P=0.045)。VEGF蛋白表达与RMS患者的分期有相关性(P=0.026),但与患者的年龄(P=0.478)、性别(P=0.944)、肿瘤大小(P=0.535)、病理亚型(P=0.399)及淋巴结转移(P=0.356)无相关性;VEGF蛋白表达阴性组与表达阳性组患者的3年总生存率分别为68.1%及31.1%(P=0.011)。COX回归分析显示,VEGF表达、肿瘤分期和肿瘤大小是RMS的独立不良预后因素(P分别为0.005、0.004和0.000),而HIF-1α尚不能作为RMS的独立不良预后因素(P=0.845)。结论:RMS组织中存在HIF-1α蛋白和VEGF蛋白的高表达,与肿瘤分期有关;HIF-1α和VEGF的表达存在相关性;VEGF的表达是RMS独立的不良预后因素。
Objective：To investigate the expressions of HIF-1α （hypoxia inducible factor-1α） and VEGF （vascular endothelial growth factor） in rhabdomyosarcoma and their clinical significance. Methods：The expressions of HIF-1α and VEGF preteins in 70 cases of rhabdomyosarcoma were detected by immunohistochemistry, and the correlations of the expressions of HIF-1α and VEGF with the clinicopathological characteristics and the prognosis were analyzed. Results：The positive-expression rates of HIF-1α and VEGF in rhabdomyosarcoma tissues were 75.7% （53/70） and 72.9% （51/70）, respectively. HIF-1α expression was positively correlated to VEGF expression （r = 0.554, P = 0.000）. HIF-1α expression was significantly associated with the factors of gender （P = 0.035）, tumor size （P = 0.016） and clinical staging （P = 0.008）, but it was not associated with the age （P = 0.555）, pathological type （P = 0.625） and lymph node metastasis （P = 0.750）. The three-year overall survival rates of HIF-1α-negative patients and HIF-1α-positive patients were 54.5% and 38.0%, respectively （P = 0.045）. VEGF expression was significantly associated with clinical stage （P = 0.026）, but it was not associated with the age （P = 0.478）, gender （P = 0.944）, tumor size （P = 0.535）, pathological type （P = 0.399） and lymph node metastasis （P = 0.356）. The three-year overall survival rates of VEGF-negative patients and VEGF-positive patients were 68.1% and 31.1% （P = 0.011）. COX regression model showed that the factors of VEGF expression （P = 0.005）, clinical staging （P = 0.004） and tumor size （P = 0.000） were independent predictors of poor prognosis of rhabdomyosarcoma. HIF-1α could not be considered as an independent prognostic factor of rhabdomyosarcoma （P = 0.845）. Conclusion：The overexpression of HIF-1α and VEGF in rhabdomyosarcoma is related to clinical staging. HIF-1α expression is associated with VEGF expression, and VEGF is an independent predictor of poor prognosis of rhabdomyosarcoma.
出处
《肿瘤》
CAS
CSCD
北大核心
2012年第8期634-638,共5页
Tumor
