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A型激酶锚定蛋白在小鼠卵母细胞减数分裂阻滞中的作用

Dynamic Anchoring of PKA via AKAPs is Essential for Meiotic Resumption of Mouse Oocytes
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摘要 目的探讨A型激酶锚定蛋白(AKAPs)在小鼠卵母细胞减数分裂阻滞中的作用。方法收集生发泡期小鼠卵母细胞,显微注射Ht31或Ht31-P,观察卵母细胞减数分裂恢复情况。结果在蛋白激酶A(PKA)持续激活的情况下,Ht31注射组能促进小鼠卵母细胞减数分裂重新启动,解除PKA引起的生发泡期阻滞,而Ht31-P对照组不能。结论 AKAPs介导的PKA正确锚定对小鼠卵母细胞第一次减数分裂阻滞至关重要。 Objective To explore the role of A-kinase anchoring proteins （AKAPs） in meiotic arrest of mouse oocytes. Methods Ht31 or Ht31-P peptide was microinjected into GV-stage mouse oocytes that were incubated in the presence of dbcAMP. The GVBD rotes were counted and the phosphorylation status of Cdc2-Tyrl5 was determined by westem blot. Results Ht31 stimulated oocyte maturation as indi- cated by GVBD and dephosphorylation of Cdc2-Tyrl5,which overcame the dbcAMP-induced arrest. In the control group, the inactive Ht31-P had no effect on GVBD,and Cdc2-Tyrl5 was phosphorylated. Conclusion PKA （protein kinase A） interaction with AKAPs is essential for the initial maintenance of meiotic arrest in mouse oocytes.
作者 王述森 张震 罗阳 张阳
机构地区 中国医科大学 中国医科大学
出处 《中国医科大学学报》 CAS CSCD 北大核心 2012年第7期588-590,共3页 Journal of China Medical University
基金 国家自然科学基金青年基金资助项目(30800392) 辽宁省教育厅高校科研计划(2008807)
关键词 卵母细胞 减数分裂阻滞 A型激酶锚定蛋白 oocytes meiotic arrest A-kinase anchoring proteins
分类号 Q291 [生物学—细胞生物学]
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参考文献11

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中国医科大学学报
2012年 第7期

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