摘要
以牛血清白蛋白与其单克隆抗体为样品，初步研究了蛋白质的非竞争性毛细管电泳免疫分析方法。混合温育可以在对20min内达到平衡，电泳分离在12min内完成。当示踪物浓度为320nmol／L时，所得到的标准曲线的线性范围为8－150nmol／L，检出限为5nmol／L，并考察了缓冲溶液的浓度、pH、分离电压等因素的影响。
The noncompetitive capillary electrophoresis based immunoassay for proteins using bovine serum albumin (BSA)and its monoclonal antibody (anti-BSA)as a model system were studied.BSA was labeled with fluorescein isothiocyanate and used as a tracer to determine anti-BSA.The binding reaction between antiBSA and the tracer reached equilibrium in less than 20 minutes,and separation between the immunocomplexes and the free tracer was achieved in 12 minutes using capillary zone electrophoresis.The linear dynamic range of the standard curve was 8~150 nmol/L,and the detection limit was 5nmol/L when a tracer concentration of 320 nmol/L was used.Influences of separation conditions such as buffer concentration,pH and separation voltage was also studied.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2000年第6期687-691,共5页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金
