摘要
目的:探讨SDZPSC 833和维拉帕米(Verapamil,VER)对MMC体外增敏作用。方法:以人宫颈癌Hela细胞和耐药亚系Hela/MMC细胞为材料,观察了PSC 833和VER对MMC体外增敏作用。结果:无毒剂量的PSC 833和VER(1~3μg/ml)可显著增加MMC的细胞毒作用且可基本克服Hela/MMC细胞对MMC 5倍的耐药,3 H_TdR实验表明,VER或PSC 833与MMC联用可增加MMC对宫颈癌细胞DNA合成的抑制,且PSC作用强于VER,光镜和电镜下形态学观察,联合用药组细胞呈明显退行性变。结论:无毒剂量的PSC 833和VER体外能基本逆转Heal/MMC细胞对MMC的耐药性,且PSC 833作用强于VER,PSC 833作为耐药修饰剂可望用于宫颈癌化疗,其临床应用前景优于VER。
Objective:To determine the reversion of mitomycin(MMC)resistance by SDZ PSC 833 and cerapamil(VER)in human cervical in vitro.Methods:The reversion of mitomycin of resistance by SDZ PSC 833 or VER was detected for human cervical cancar cell(Hela)and its resistant subline Hela/MMC in virto.Results:Both nontoxin doses of PSC 833 and VER might enhance the cytotoxicity of MMC in Hela and Hela/MMC3μg/ml of PSC 833 and VER could result in almost complete or partial reversion of MMC-resisistance of Hela/MMC 3H-TdR incorporation test indicated that PSC 833 or VER enhanced the inhibition of DNA synthesis in Hela and Hela/MMC cell.Moreover the effect of PSC 833 on reversing drug restance was better than VER in vitro.Conclusions:PSC 833 and VER can overcome mitomycin resistance of Hela/MMC in vitro.PSC 833 will be a better candidate for reversing multidrug resistant thanverapamilin clinic.
作者
李萍
陈红
陈惠祯
吴绪峰
杨庆忆
刘诗权
Li Ping;Chen Hong;Chen Huizhen(Department of Obstetrocs and Gynecology,Second Affiliated Hospital,Hubei Medical University,Wuhan 430071,China)
基金
湖北省教委资助课题
关键词
子宫颈肿瘤
药物耐受性
丝裂霉素C
cervix neoplasms
mitomycin
drug resistance
reversion
cyclosporin
verapamil
