摘要
目的:研究P15基因通过甲基化失活在骨髓增生异常综合征(MDS)发病中的作用。方法:用甲基化敏感的限制性核酸内切酶消化,结合聚合酶链反应(PCR)技术。结果:20例MDS患者中发现有9例(45%)存在P15基因高度甲基化,且多为高危MDS(RAEB、RAEB_(t))(P<0.05)。结论:P15基因可通过甲基化而失活,可能与部分MDS的发生及发展有关。
Objective:To explore the role of inactivation of P15 gene by means of hypermethylation in the pathogenesis of MDS.Methods:20 cases of MDS patients were studied using methylation sensitive restriction enzyme digestion and polymerase chain reaction(PCR)technique.Results:Hypermethylations of P15 gene were found in 9/20(45%)of the MDS patients,and most of them were high-risk MDS(RAEB,RAEB-t)compared with the low-risk subtype(RA,RAS)(P<0.05).Conclusion:The results indicate that P15 can be inactivated through hypermethylation in MDS,and may be involved in the initiation and developing of some MDS patients.
作者
黄建英
曾宪昌
陈飞
任波
Huang Jianying;Zeng Xianchang;Chen Fei(Department of Hematolology,Second Affiliated Hospital,Hubei Medical University,Wuhan 430071,China)
基金
湖北省自然科学基金资助课题(No95J32)
关键词
骨髓增生异常
综合征
DNA甲基化
P15基因
myelodysplastic syndromes/ET
genes,suppressor,tumor
DNA methylation
polymerase chain reaction
