摘要
为更好地了解肾细胞癌的发病机制,应用免疫组化技术方法,检测47例肾细胞癌中bcl_2、ki_67和P53蛋白表达,并分析其临床意义。结果:bcl_2、ki_67和P53表达在不同细胞类型中无明显差异,病理分级中,bcl_2阳性率随分级升高而降低,ki_67和P53表达率则随分级升高而增高;不同临床分期中,ki_67和bcl_2表达率无统计学差别,但P53阳性率在临床I期明显高于其它各期;bcl_2、ki_67和P53阳性和阴性标本中,术后5年生存率差异无显著性,但ki_67阳性组5年生存率明显降低。结论:bcl_2、ki_67和P53表达在RCC发生中有一定作用,但与RCC细胞分类无关,bcl_2表达在细胞癌变早期有一定作用,ki_67表达率升高与细胞恶性程度和预后不良有关,P53蛋白表达多出现于RCC临床早期阶段,故有一定诊断意义.
In order to study the cause of renal cell carcinoma(RCC),bcl_2,ki_67 and P53 expressions were detected by immunohistochemistry in 47 cases.In different cell types,no significant differences were observed in these three proteins.In Fuhrman grades,bcl_2 positive rates were decreased as grades increased,but in both ki_67 and P53,the higher grade,the higher rates.There were no significnat differences among robson stages for bcl_2,ki_67 expressions.However,in stage one,P53 positive rate was higher than those in other stages.Also no significant differences were found between positive and negtive groups of bcl_2 and P53 for 5_year survival rate.But in ki_67 positive group,the survival rate was lower.The results show:bcl_2,ki_67 and P53 expression were not affected by RCC celluar types;bcl_2 protein plays a role mainly in early cell malignancy;ki_67 is connected with malignant and prognosis of the tumour,P53 expression occurs early clinic stage,which has the valur in diagnosis.
作者
吴小燕
徐联芳
曾俊
龚玲玲
熊永炎
Wu Xiaoyan;Xu Lianfang;Zeng Jun(Department of Kidney Disease,Second Affiliated Hosital,Hubei Medical University,Wuhan 430071,China)
