摘要
目的 :克隆、表达HPV16湖北株E7基因并探讨E7蛋白的免疫学性质。方法 :应用基因克隆技术克隆、表达HPV16E7基因 ,纯化E7蛋白并免疫动物 ,用ELISA检测动物血清中的特异性E7抗体 ,进而评估E7蛋白的抗原性。结果 :融合型的E7蛋白产量可达细胞总蛋白量的 30 % ;ELISA检测表明动物体内产生了特异性E7抗体。结论 :HPV16湖北株E7蛋白可通过基因克隆方法制备 。
Objective:To study the immunogenicity of E7 protein and gene of HPV16 (HB strain).Methods:To clone and express E7 gene of HPV16 with gene clone technique.To pruify E7 protein and inoculate mice.The reactivity of special E7 antibody in sera was detected by ELIAS to evaluate antigenicity of E7 protein.Results:The fusion protein LacE7 occupied 30% of the total E.coli protein.Mice inoculated with E7 fusion protein were elicited spcial E7 antibody. Conclusions:E7 protein of HPV16 (HB strain) could be prepared by gene clone technique.HB-E7 protein displayed antigenicity of standard E7 protein.
基金
湖北省科委"八五"攻关课题资助!批准号:922P1701
关键词
乳头瘤病毒
E7基因
克隆
抗原性
基因表达
oncogene proteins,viral
E7 gene
papillomaviruses
gloning,molecular
