摘要
目的分析长期饲喂n-3多不饱和脂肪酸(PUFA)高脂饮食对大鼠胰岛素敏感性的影响。方法雄性SD大鼠随机分为2组,对照组(NC)给予普通日粮,高脂组(TUF)给予富含n-3PUFA的高脂日粮。喂养16周,记录采食量及体质量,用高胰岛素—正常血糖钳夹技术评价大鼠胰岛素敏感性。结果 TUF组体质量与NC组没有统计学差异(P>0.05),基础血糖及胰岛素与对照组没有统计学差异(P>0.05),葡萄糖输注率(GIR)显著低于NC组(P=0.031)。结论长期过量摄入n-3PUFA导致大鼠胰岛素抵抗。
The n-3 Polyunsaturated fatty acids（PUFA） are increasingly consumed as food supplements;however,the side effects of it,especially at high doses,remain unclear.The aim of this study was to analyze insulin sensitivity in SD rats fed with high-fat diet rich in n-3 PUFA.Normal male SD rats were randomly divided into two groups,including normal control group（NC） and high n-3PUFA formula diet group（TUF）.Rats were fed for 16 weeks,diet intake and weight were recorded,insulin sensitivity was estimated by euglycemic-hyperinsulinemic clamp.By the end of the experiment,weight was similar in the two groups,fasting plasma glucose and plasma insulin in TUF group were not significantly different compared to NC group（P〉0.05）,GRI in TUF was obviously lower than that in NC group（P=0.031）.In conclusion,Overdosage intake of n-3PUFA significantly decreased insulin sensitivity in rat.
出处
《实验动物科学》
2012年第3期31-33,共3页
Laboratory Animal Science
基金
国家自然科学基金面上项目(No.31071986)
军事医学科学院实验动物中主青年基金(No.QN2010-005)
