摘要
目的 :建立一阶导数紫外光谱法测定盐酸芬氟拉明片的含量及含量均匀度 ,以增加质量控制方法。方法 :用水作溶剂 ,在 2 80与 2 2 0nm波长范围内测定一阶导数光谱 ,以一阶导数光谱在2 65nm与 2 69nm波长处峰 -谷间的振幅D为定量依据 ,用对照品对照法计算含量及含量均匀度。结果 :盐酸芬氟拉明浓度在 0 0 5～ 0 4mg/ml之间与—阶导数峰—谷间的振幅呈良好的线性关系 ,相关系数r=0 9999,6次的平均加样回收率为 1 0 0 6% ,RSD =0 7%。结论 :本法准确性和重现性好 ,操作简便。
OBJECTIVE:To provide a useful way of quality control,a first order derivative spectrophotometric method had been developed to determine the content and uniformity of content of fenfuramine hydrochloride tables.METHODS:Water was selected for solvent.The first derivative spectrum was obtained by scanning from 280 to 220nm.The distance D of the derivative peak at 265nm and valley at 295nm was used as quantitative basis.The assay was calculated by comparing the peak-valley distance of sample with standard.RESULTS:There was a good linear relationship between fenfuramine hydrochloride concentration in the range of 0.05～0.40mg/ml and the peak-valley distance of derivative spectrum with r =0.9999.The average recovery form sample was 100.6%. RSD =0.7%( n =6).CONCLUSIONS:The proposed method is simple,accurate and reproducible.
出处
《药学实践杂志》
CAS
2000年第3期154-156,共3页
Journal of Pharmaceutical Practice
关键词
一阶导数紫外光谱法
盐酸芬氟拉明片
含量均匀度
first order deviative spectrophotometry
fenfuramine hydrochloride tablets
quantitative analysis
uniformity of content
