摘要
目的 :建立人肝癌裸鼠皮下 -肝原位移植瘤模型。方法 :先用组织学完整的新鲜人肝癌组织接种于裸鼠皮下 ,形成皮下移植瘤 ,然后用此移植瘤组织再接种于裸鼠肝内 ,建立肝原位移植瘤模型 (间接肝原位移植瘤模型 ) ,并将其与直接肝原位移植瘤模型、皮下移植瘤模型和腹腔内移植瘤模型作比较。 结果 :建立的间接肝原位移植瘤模型能稳定传代 ,移植瘤存活率达 10 0 % ,自发转移率达 5 7.8%。与直接肝原位移植瘤模型比较 ,该模型移植瘤存活率明显高于后者 (35 .6 % ,P<0 .0 1) ;与皮下移植瘤模型和腹腔内移植瘤模型比较 ,该模型局部侵袭率 (10 0 % )和自发远处转移率 (5 7.8% )明显高于皮下移植瘤模型 (14.5 % ,P<0 .0 1;37.1% ,P<0 .0 5 )和腹腔内移植瘤模型 (18.0 % ,P<0 .0 1;39.3% ,P<0 .0 5 )。 结论 :人肝癌裸鼠皮下 -肝原位移植瘤模型具有高存活率、高侵袭率和高转移率的优点 。
Objective: To establish the orthotopic transplantation tumor model from the subcutaneous model of human hepatocellular carcinomas (HCC) in nude mice. Methods: Fresh specimens of HCC were transplanted to form subcutaneous transplantation tumor in nude mice using histologically intact tissue. Then the orthotopic transplantation tumor model was established by injecting the subcutaneous transplanted tumor into liver (indirect orthotopic model). It was compared with the direct orthotopic model, subcutaneous model and peritoneal cavity model for HCC in nude mice. Results: The indirect orthotopic model propagated stably. The survival rates and spontaneous metastatic rates of the transplantation tumor were 100% and 57.8%, respectively. The model had higher survival rates (100%) than direct orthotopic model (35.6%, P <0.01). It also had higher local invasion rates (100%) and spontaneous metastatic rates (57.8%) than the subcutaneous model (14.5%, P <0.01; 37.1%, P <0.05) and the peritoneal cavity model (18.0%, P <0.01; 39.3%, P <0.05). Conclusion:The model is an ideal instrument for study of the metastatic mechanism and screen of anti metastatic drugs.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第5期456-459,共4页
Academic Journal of Second Military Medical University
