摘要
目的 :了解血管内皮生长因子 (VEGF)基因治疗兔下肢缺血模型后新生血管和侧支循环形成状况。 方法 :构建h VEGF1 6 5 的真核表达载体 ,通过直接肌肉注射将 h VEGF1 6 5 基因转染缺血部位肌细胞 ,经颈总动脉插管行下肢血管造影。结果 :VEGF治疗组在质粒转染后 2和 4周动脉血管造影显示 ,新生血管数目和侧枝血管形成较对照组明显增加。 结论 :肌细胞能够吸收裸露的 VEGF c DNA质粒并获得局部高效表达 。
Objective:To study the angiogenesis and collateral circulation formation in a rabbit model of hindlimb ischemia after gene therapy of VEGF. Methods:The hVEGF 165 cDNA was cloned into eukaryotic expression vector pcDNA 3. The pcDNA 3/hVEGF 165 was administered intramuscularly, and angiography was performed through the common carotid artery. Results:The angiography was performed 2 and 4 weeks after the initiation of therapy. In VEGF treated animals, neovessels and collateral artery development were more evident compared with controls. Conclusion:Naked DNA of VEGF can be absorbed by muscle cells, and expressed efficiently and locally, to stimulate angiogenesis and collateral circulation in vivo .
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第5期447-449,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金资助项目!(39780025)
关键词
侧支循环
血管内皮生长因子
基因治疗
下肢缺血
collateral circulation
vascular endothelial growth factor
gene therapy
hindlimb ischemia
