摘要
建立了采用超高效液相色谱-串联质谱仪(UPLC-MS/MS)检测不同动物样品中螺虫乙酯及其主要代谢物残留量的方法,并研究了螺虫乙酯在大鼠体内的吸收与代谢。样品中螺虫乙酯及其主要代谢物经甲醇提取及C18固相萃取(SPE)柱净化后,用UPLC-MS/MS检测。结果显示,所建立方法快速、灵敏,每个样品上机检测仅需3 min,方法的最低检出浓度(LOQ)为0.005 mg/kg。对按照每千克体重每日250 mg剂量染毒28 d后的大鼠体内螺虫乙酯残留量的检测结果表明:螺虫乙酯在睾丸、肝脏、肺、肾、心脏、血浆等器官和组织中的残留量较低,平均在0.012～0.025 mg/kg之间,且分布差异不显著,而脂肪和肌肉中螺虫乙酯的残留量显著低于睾丸和肝脏中的残留量(P<0.05);螺虫乙酯可被大鼠吸收并转化为代谢物醇(spirotetramat-enol),各器官和组织中代谢物残留量差异显著(P<0.05),其浓度分布从高到低依次为肝脏>肾>血浆>肺>心脏>睾丸>脂肪>肌肉。
A method was developed to determine the residue of spirotetramat and its metabolite in various rat organ by ultra performance liquid chromatograph equipped with triple quadrupole mass spectrometer（UPLC-MS/MS）.And the distribution and metabolism of spirotetramat in rat were studied.The spirotetramat and metabolite in rat organs were extracted with methanol,cleaned up by solid phase extraction（SPE）,and detected by UPLC-MS/MS.The results showed that it took only 3 minutes for sample analysis by MS,and the limits of quantitation（LOQ） were 0.005 mg/kg.The spirotetramat and metabolite in organs of rat given 250 mg/（kg bw·d） of spirotetramat were analyzed using the method.The test indicated that there were no significant difference of spirotetramat residue among testicle,liver,lung,kidney,heart and plasma and low levels ranged 0.012-0.025 mg/kg.The spirotetramat residues in fat and muscle were lower（P〈0.05） than that in testicle and liver.Spirotetramat can be metabolized easily in body and the main metabolite is spirotetramat-enol（BYI08330-enol）.Significant differences of spirotetramat-enol concentration have found among various organ of rat.The rank by high-to-low was liver〉kidney〉plasma〉lung〉heart〉testicle〉fat〉muscle.
出处
《农药学学报》
CAS
CSCD
北大核心
2012年第4期417-422,共6页
Chinese Journal of Pesticide Science
