摘要
本实验采用大鼠体表30%烧伤模型;观察烧伤组、烧伤用药(超氧化物歧化酶SOD和过氧化氢酶CAT)组及正常对照组的小肠丙二醇(MDA)含量、小肠粘膜的Na-KATP酶活性、小肠湿/干重比值和小肠对亮氨酸吸收率的变化。结果表明,烧伤后小肠粘膜Na-KATP酶活性降低,肠含水量增加,和亮氨酸吸收率下降,均与小肠脂质过氧化损伤有关。应用SOD+CAT后,在降低烧伤后小肠MDA含量的同时,减轻了肠道水肿,增加了小肠粘膜Na-KATP酶活性,提高了小肠对亮氨酸的吸收率。
The purpose of this study was to investigate the dynamic changes of the level of lipid peroxidation products (malonaldehyde, MDA) of intestine, intestinal water, Na-K ATP- ase activity of intestinal mucosa and the intestinal leucine absorption rate of rats subjected to 30% Ⅲ° burns. The results showed that the value of the intestinal MDA was higher, the Na-K ATPase activity of the intestinal mucosa reduced markedly. the wet/dry ratio of intestinal weight was increased significantly and the intestinal leucine absorption rate in vivo was distinctly reduced postburn. However. the content of intestinal MDA and the wet/dry ratio of intestine weight was significantly reduced, and the Na-K ATPase activity and leucine absorption rate was increased in burn rats treated with SOD and CAT than in untreated burn rats. These results strongly suggested that lipid peroxide may play an important role in the impairment of leucine absorption rate of intestine after burns, and the edema and reduced Na-K ATPase activity of intestinal mucosa resulted from the in- creased lipid peroxide might take active parts impairing the intestinal absorption.
