摘要
背景:研究证实,间充质干细胞移植进入糖尿病患者体内后,疗效受多种因素影响,其中血糖、血脂起着关键作用。目的:模拟糖尿病患者体内高糖高脂环境,进一步验证高糖高脂对体外培养脐带间充质干细胞的影响。方法:葡萄糖和软质酸不同浓度分别体外干预人脐带间充质干细胞,分为对照组、低糖组、低脂组、低糖低脂组、高糖组、高脂组及高糖高脂组。干预48h后通过CCK-8方法酶标仪测定细胞增殖,Annexin-V/PI方法流式细胞仪检测各组细胞的凋亡情况。结果与结论:在高糖高脂的条件下,短期培养后细胞增殖及凋亡明显改变,与对照组相比,人脐带间充质干细胞增殖率明显减低(P<0.01)、凋亡率明显增高(P<0.01),且随着血糖血脂浓度的增加,人脐带间充质干细胞增殖率明显受到抑制,凋亡率明显上升;低糖低脂组和高脂组均抑制细胞增殖(均P<0.05),促进细胞凋亡(均P<0.01),高糖则促进人脐带间充质干细胞生长(P<0.01)。结果提示,糖尿病患者体内处于高糖高脂状态,将抑制脐带间充质干细胞的生长。在间充质干细胞移植之前,控制患者血糖血脂水平,将更有利于干细胞在体内发挥作用。
BACKGROUND： Several studies have confirmed that the curative effects of mesenchymal stem cell transplantation into diabetes patients in vivo are influenced by many factors, in which blood glucose and lipids play a key role. OBEJECTIVE： To simulate in vivo environment of high glucose and high lipid and further validate the effects of high glucose and high lipid on umbilical cord mesenchymal stem cells cultured in vitro. METHODS： Human umbilical cord mesenchymal stem cells were cultured using cell culture medium containing different concentrations of glucose and palmitic acid. Then the cells were divided into seven groups： control, low glucose, low lipid low glucose＋low lipid, high glucose, high lipid, and high glucose＋high lipid. After intervention for 48 hours, cell proliferation was measured by CCK-8 method and cell apoptosis was detected by Annexin-V/PI method. RESULTS AND CONCLUSION： Under the condition of high glucose and high lipid, cell proliferation was significantly decreased （P 〈 0.01 ）, while cell apoptosis was significantly increased （P 〈 0.01 ） after short-term culture compared with the control group. With increasing blood glucose and blood lipid concentration, cell proliferation was significantly inhibited, and cell apoptosis was significantly increased. In the low glucose ＋ low lipid group and high lipid group, cell proliferation was inhibited （both P 〈 0.05） and cell apoptosis was increased （both P 〈 0.01 ）. High glucose promoted the growth of human umbilical cord mesenchymal stem cells （P 〈 0.01 ）. Results showed that diabetes patients exhibited a high glucose and high lipid in vivo environment, which inhibit the growth of human umbilical cord mesenchymal stem cells. Prior to transplantation of mesenchymal stem cells, control of patient＇s blood glucose and lipid level would benefit for the functioning of transplanted stem cells.
出处
《中国组织工程研究》
CAS
CSCD
2012年第27期5001-5005,共5页
Chinese Journal of Tissue Engineering Research
