摘要
目的:探讨双酚A(BPA)暴露水平以及BPA产生生殖毒性的潜在机制。方法:采用气相色谱质谱联用法检测新生儿(贵屿区153例为暴露组,濠江区47例为对照组)脐带血BPA浓度;实时定量RT-PCR法检测新生儿胎盘组织KiSS-1基因表达量。结果:暴露组脐带血BPA浓度(中位数6.4μg/L)显著高于对照组(2.8μg/L),暴露组胎盘组织KiSS-1基因相对表达量(中位数0.5)同样显著高于对照组(0.2),BPA浓度与KiSS-1表达量正相关(P<0.05);男性组该相关性更为显著(P<0.01)。结论:贵屿区人群已处于较高的BPA暴露水平,BPA有可能通过影响亲吻素的正常表达而干扰生殖内分泌系统。
Objective： To investigate the level of bisphenol A（BPA） exposure and potential mechanism of BPA repro- ductive toxicity. Methods： 02-MS was used to determine the umbilical cord blood BPA concentration of neonates （ 153 from Guiyu as exlx^ure group and 47 from Haojiang as control group）. The relative expression of K/SS-1 gene in placenta was determined by real time RT-PCR. Results： The BPA concentration（mean 6.4 ttg/L）in the exposure group was sig- nificantly higher than that in control group（mean 2.8 ttg/L）, The relative expression of K/SS-l（mean 0.5）in the expo- sure group was also significantly higher than that in control group（mean 0.2）. In addition a positive association had been found between BPA concentration and expression of K/SS-1 （ P 〈 0.05） and such association in male neonates was more significant（ P 〈 0.01）. Conclusion： People living in Guiyu are exposed to higher BPA level. BPA may affect the repro- ductive system by disturbing the expression of kisspeptin encoded by K/SS-1 and breaking the endocrine balance.
出处
《汕头大学医学院学报》
2012年第2期72-74,共3页
Journal of Shantou University Medical College
基金
广东省自然科学基金资助项目(10151503102000008)
