摘要
In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for 18-111 tubulin （neuronal marker）, and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for 04 （oligodendrocytic marker）. At 14 days, there were 5.6% nestin-, 9.6% 13-111 tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibdllary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.
In this study, cells from the cerebral cortex of fetal rats at pregnant 16 days were harvested and cultured with 20 μg/L neurotrophin-3. After 7 days of culture, immunocytochemical staining showed that, 22.4% of cells were positive for nestin, 10.5% were positive for 18-111 tubulin （neuronal marker）, and 60.6% were positive for glial fibrillary acidic protein, but no cells were positive for 04 （oligodendrocytic marker）. At 14 days, there were 5.6% nestin-, 9.6% 13-111 tubulin-, 81.1% glial fibrillary acidic protein-, and 2.2% O4-positive cells. In cells not treated with neurotrophin-3, some were nestin-positive, while the majority showed positive staining for glial fibdllary acidic protein. Our experimental findings indicate that neurotrophin-3 is a crucial factor for inducing neural stem cells differentiation into neurons and oligodendrocytes.
基金
The National Natural Science Foundation of China，Medical Scientific Research of Zhejiang Province
