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pH对重组CHO细胞生长及产物EPO表达的优化

Optimization of growth of reconstructed CHO cells and expression of its product EPO
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摘要 目的探索唾液酸化程度高且EPO表达量高的最优DH。方法考察不同pH对细胞生长、EPO产量和EPO唾液酸含量的影响。结果pH7．10～7．25EPO表达量可达10388IU／ml，唾液酸可达11．5mol／molEPO，纯化收率达17．9％。结论综合细胞维持时间、EPO表达量、纯化收率、唾液酸含量等因素，选择pH7．10～7．25为CHO细胞表达EPO的最优pH，为优化工业化生产EPO提供基础。 ObJective To explore the optimal pH value for over-expression of erythropoietin with high level of sialic acidification. Methods The effects of different pH values on cell growth, yield of EPO, and content of EPO sialic acid were obserred. Results Level of EPO expression could reach 10 388 IU/ml, sialic acid up to 11.5 mol/molEPO, and purification yield up to 17.9% as the pH value was between 7.10 to 7,25. Conclusions 7.10 to 7.25 can be the optimal pH value by a comprehensive assessment of the factors of level of cell maintenance, expression of EPO, purification yield, and sialic acid content, which provides a basis for optimization of the industrial production of EPO.
作者 吴园园 盛光阳 张涤平 吴建祥 黎雄辉 黄俊龙
机构地区 深圳赛保尔生物药业有限公司
出处 《国际医药卫生导报》 2012年第15期2184-2186,共3页 International Medicine and Health Guidance News
关键词 PH CHO细胞 EPO 细胞培养 pH CHOcells Erythropoietin Cell culture
分类号 R96 [医药卫生—药理学]
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参考文献10

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国际医药卫生导报
2012年 第15期

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