摘要
目的:观察山茶籽油对去卵巢大鼠骨组织和骨代谢的影响,为山茶籽油防治绝经后骨质疏松症提供实验依据。方法:6月龄健康雌性SPF级SD大鼠80只,分成4个实验组(每组20只):①对照组、②去卵巢组、③去卵巢+己烯雌酚组(DTS)、④去卵巢+山茶籽油组(CSO)。治疗组用药:去卵巢+己烯雌酚组,皮下注射己烯雌酚,治疗剂量为300 ug/kg,2次/周,连续12周;去卵巢+山茶籽油组,采用经口灌胃方式进行用药(山茶籽油),治疗剂量为5 ml/kg,每天1次,连续12周。各实验组在第12周,麻醉大鼠后取左心血,用于测量血清雌二醇、黄体酮、促卵泡激素、黄体生成素及血清钙、磷和碱性磷酸酶;再将大鼠处死后,取股骨近端切片观察骨组织结构。数据进行统计学分析。结果:与对照组比较,去卵巢组的血清钙、磷和雌二醇明显降低(P﹤0.01),而血清碱性磷酸酶、促卵泡激素明显升高(P﹤0.01),骨组织切片呈骨质疏松的病理改变;与去卵巢组比较,2个治疗组的血清钙、磷和雌二醇明显升高(P﹤0.01),而血清碱性磷酸酶、促卵泡激素明显降低(P﹤0.01),骨组织切片骨质疏松的病理改变得到明显改善。结论:山茶籽油对去卵巢大鼠的骨质疏松症有治疗作用,与己烯雌酚治疗效果相近。
Objective： To observe the effect of tea camellia seed oil on bone tissue and bone metabolism of ovariectomized rats, provide an experimental basis for preventing and treating postmenopausal osteoporosis with tea camellia seed oil. Methods ： A total of 80 six - month healthy female SD rats were divided into four groups, 20 rats in each group ： control group, ovariectomized model group, ovariecto-mized model plus diethylstilbestrol group, and ovariectomized model plus tea camellia seed oil group. The rats in ovariectomized model plus diethylstilbestrol group were treated with subcutaneous injection of diethylstilbestrol for successive 12 weeks, the therapeutic dose was 300 μg/kg, twice a week; the rats in ovariectomized model plus tea camellia seed oil group were treated with pouring tea camellia seed oil into stomach for successive 12 weeks, the therapeutic dose was 5 ml/kg, once a day. The rats in the four groups were anesthetized at 12 weeks, then the blood samples of left heart were obtained to detect the levels of serum estradiol, progesterone, follicle - stimulating hormone （FSH）, luteinizing hormone （LH） , calcium, phosphorus, and alkaline phosphatase; then the rats were killed, proximal femoral sections were prepared to observe the structure of bone tissue; all the data were analyzed statistically. Results ： Compared with control group, the lev- els of serum calcium, phosphorus, and estradiol in ovariectomized model group decreased significantly （P 〈0. 01 ）, while the levels of alka- line phospbatase and FSH increased significantly （P 〈 0.01 ） , bone sections showed obvious pathological changes of osteoporosis; compared with ovariectomized model group, the levels of serum calcium, phosphorus, and estradiol in ovariectomized model plus diethylstilbestrol group and ovariectomized model plus tea camellia seed oil group increased significantly （P 〈 0. 01 ）, while the levels of alkaline phosphatase and FSH decreased significantly （ P 〈 0. 01 ）, bone sections showed that the pathological changes of osteoporosis were improved significantly. Conclusion： Tea camellia seed oil had therapeutic effect for osteoporosis in ovariectomized rats, and the effect was similar to that of diethylstilbestrol.
出处
《中国妇幼保健》
CAS
北大核心
2012年第23期3646-3649,共4页
Maternal and Child Health Care of China
基金
广西自然科学基金〔2010GXNSFA013268〕
广西中医药管理局科研课题〔gzzc1049〕
关键词
山茶籽油
己烯雌酚
去卵巢大鼠
骨质疏松症
Tea camellia seed oil
Diethylstilbestrol
Ovariectomized rat
Osteoporosis
