摘要
本文采用微量细胞毒试验检测27只实验性溃疡性结肠炎(下称资结)大白鼠的周圈血淋巴细胞(PBL)和结肠粘膜淋巴细胞(LCM)以及13例溃结病人的PBL的细胞毒活性。溃结组淋巴细胞毒指数明显高于正常组(P<0.001)。在溃结细胞毒测定过程中,淋巴细胞经胰酶处理后细胞毒活性显著下降,如再经自身灭活血清温育1小时则可恢复。用限酶处理靶细胞对细胞毒无影响。结果表明溃结细跑毒是抗体依赖性细跑介导细胞毒。
The SeThe study was undertaken with microcytotoxicity assay to evaluate the cytotoxicityof peripheral blood lymphocytes(PBL)and lymphocytes in colonic mucosa(LCM)in 27 ex-perimental rats and the cytotoxicity of PBL.in 13 patients with ulcerative colitis for autologouscolonic epithelial cells(CEC).Control groups were designed for corresponding study groups.The results shówed that cytotoxicity indices of PBL,and LCM of experimental rats were41.63±3.8%and 38.40±2.1%respectively(8.2±1.91%and 7.0±1.76%respectively incontrol group,P<<0.001).The cytotoxicity index of PBL,of patients was 39.77±14.9%(11.3±2.64%in control group,P<0.001).The cytotoxicity was markedly reduced by trypsinization of the lymphocytes and it wasrestored if the trypsinized cells were exposed to heat-inactivated autologous plasma.Trypsin-ization of the target cells had no effect on eytotoxicity.It is suggested that this phenomenonis a form of antibodydependent cell-mediated cytotoxicity(ADCC).We consider that thepathogenesis of ulcerative colitis may be related to cytotoxicity.
作者
黄梅芳
傅苓
Huang Mei-fang(The Second Affitiated Hospital,Hubei Medical College)
出处
《中华消化杂志》
CAS
CSCD
北大核心
1990年第5期268-270,共3页
Chinese Journal of Digestion
