摘要
通过对分离草鱼（ Ｃｔｅｎｏｐｈａｒｙｎｇｏｄｏｎ ｉｄｅｌｌａ） 外周血淋巴细胞技术的探索，发现用相对体积质量为１ ．０８０ ～１ ．０８５ 的Ｆｉｃｏｌｌ－ Ｕｒｏｇｒａｆｉｎ 分层液可将草鱼外周血中淋巴细胞１００ ％ 地分离出来，这对进一步研究鱼类淋巴细胞的结构、功能具有重要意义。同时测得草鱼各类血细胞的相对体积质量：淋巴细胞为１ ．０７７ ±０ ．００２ ，粒细胞为１ ．０９６ ±０ ．００３ ，红细胞为１ ．１１１ ±０ ．００２ 。
The separation of lymphocytes from peripheral blood of grass carp was performed with the mixture of Ficoll-Urografin solution. The results showed that about 100 percent of the lymphocytes was isolated with the mixture at density from 1.080 to 1.085.The mixture was made from 6% Ficoll and 34% Urografin. The observation of serum layer, lymphocyte layer,granulocyte layer,erythrocyte layer and layering solution layer showed that (1)there were only a few broken erythrocytes in the serum;(2) the granulocyte layer was too thin to be sampled;(3) there was no cell in the layering solution layer;(4)in the lymophocyte layer the lymphocytes made up 80%～91%,and the monocytes made up 5%～20 %. The densities of some kinds of cells in the blood of all separation layers were:lymphocyte 1.077±0.002,granulocyte 1.096±0.003,erythrocyte 1.111±0.002 and serum 1.070±0.001. This separating technique can be used to study the structure and function of fish lyophocytes.
出处
《中国水产科学》
CAS
CSCD
1999年第4期10-12,共3页
Journal of Fishery Sciences of China
关键词
鱼类
外周血
淋巴细胞
分离技术
免疫学
fish, peripheral blood,lymphocyte,technique of separation
