摘要
目的通过体外培养人脐静脉内皮细胞(HUVECs)并加醛固酮(ALD)诱导,观察氧化低密度脂蛋白受体-1(LOX-1)mRNA表达的影响。方法体外培养的HUVECs按细胞不同处理方式分为6组:阴性对照组(1640培养基,A组)、不同浓度ALD组(10、100、1 000 nmol/L,分别为B、C、D组)、ALD+LOX-1受体阻滞剂多聚肌苷酸(Poly I)组(E组)、ALD+螺内酯组(F组)加入HUVECs中共孵育24 h,RT-PCR的方法测定HUVECs中LOX-1mRNA的表达。结果用不同浓度的ALD(10、100、1 000 nmol/L)与HUVECs培养24 h后,同A组相比LOX-1mRNA表达呈浓度依赖性增加(P<0.05);而在用螺内酯(1μmol/L)与1 000 nmol/L的ALD共培养24 h或Poly I(250 mg/L)与HUVECs预先作用2 h后再加入1 000 nmol/L的ALD共培养24 h,同D组相比E,F组LOX-1mRNA的表达明显减少(P<0.05)。结论ALD可以促进培养的HUVECs LOX-1基因的表达,而其拮抗剂螺内酯可以抑制LOX-1mRNA的表达,因此ALD的致动脉粥样硬化(AS)作用可能与LOX-1有关,而螺内酯则可能通过此途径发挥抗AS作用。
Objective To investigate the effects of spirolactone（Spiro） on the mRNA expression of lectin-like oxidized low density-lipoprotein receptor 1（LOX-1） in cultured human umbilical vein endothelial cells（HUVECs） by aldosterone（ALD）.Methods The cultured HUVECs were divided into six groups： the negative control group（group A）,treated with different various concentrations of aldosterone group（10,100,1 000 nmol/L,namely group B,C,D）,the ALD ＋ polyinosinic acid（the chemical inhibitors of LOX-1） group（group E）,the ALD ＋ Spiro group（group F）,group E was pretreated with polyinosinic acid（250 mg / L） for 2 h and then both group E and group F（treat with Spiro 1 μmol / L） stimulated with ALD for 24 h,LOX-1 mRNA was detected by RT-PCR.Results LOX-1 mRNA expression in group D was obviously upregulated than that of group A（P 〈 0.05）.However,in the presence of Spiro or pretreatment with polyinosinic acid almost reduced these effects（P 〈 0.05）.LOX1 mRNA expression in group F was not significantly different compared with pretreatment with polyinosinic acid（P 〉 0.05）.Conclusion This study demonstrates that ALD can promote LOX-1mRNA expression in human endothelial cells and Spiro can inhibite these effects,and Spiro may play a role of anti-atherosclerotic therapy through this way.
出处
《安徽医科大学学报》
CAS
北大核心
2012年第8期911-914,共4页
Acta Universitatis Medicinalis Anhui
关键词
醛固酮
螺内酯
脐静脉内皮细胞
动脉粥样硬化
血凝素氧化低密度脂蛋受体-1
aldosterone； spirolactone； umbilical vein endothelial cell； atherosclerosis； lectin-like oxidized low density-lipoprotein receptor 1
