摘要
目的观察小鼠胚胎肾脏发育过程中细胞坏死性凋亡的超微结构变化。方法应用电子显微镜技术对不同胚龄(E12、14、16、18d)胎鼠(各3例)的肾脏的细胞坏死性凋亡进行系统观察。结果细胞坏死性凋亡确实出现在胚胎肾脏中,它同时具有坏死的细胞质和凋亡的细胞核。细胞肿胀,细胞膜破溃,细胞器也肿胀空泡化。核染色质高密度固缩,边聚,多呈月牙状。细胞凋亡被发现的机会大于坏死性凋亡和细胞胀亡。结论细胞坏死性凋亡与细胞凋亡及细胞胀亡共同构成了小鼠胚胎肾脏发育过程中的程序性细胞死亡,但坏死性凋亡和胀亡为辅,而以细胞凋亡为主。
Objective To observe the uhrastructural chang of necroptosis during renal development in mice. Methods Renal neeroptosis of three mice aged at embryonic days （E）12,14,16,18 was examined by using electron microscopy. Results There was necroptosis, or programmed necrosis, in the developmental embryonic kidney tissue. EM observations demonstrated that cell necroptosis was similar to cytoplasm appearances of cell necrosis and nuclei of cell apoptosis. Those appearances were swelling cytoplasm with disrupted plasma membrane, and nuclei with shrinkage and margination of ehromatin. Conclusion Programmed cell death is composed of necroptosis, oncosis and apoptosis during renal development and apoptosis is more principal.
出处
《解剖学报》
CAS
CSCD
北大核心
2012年第4期549-552,共4页
Acta Anatomica Sinica
