摘要
The entire interaction process between anti-mouse immunoglobulin-G (IgG) and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference (OIRD) method.We achieved the two-dimensional scanning images of OIRD Re{△ p △s } intensities before and after interactions as well as the curves of reaction dynamic processes.The experimental results suggest that OIRD method not only can label-free distinguish the different concentrations of proteins and detect biological microarrays,but also can real-time monitor the kinetic processes of biomolecular interactions.
The entire interaction process between anti-mouse immunoglobulin-G （IgG） and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference （OIRD） method. We achieved the two-dimensional scanning images of OIRD Re{△p-△s} intensities before and after interactions as well as the curves of reaction dynamic processes. The experimental results suggest that OIRD method not only can label-free distinguish the different concentrations of proteins and detect biological microarrays, but also can real-time monitor the kinetic processes of biomolecular interactions,
基金
supported by the National Basic Research Program of China(2007CB935701)
