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浮游生物PCR检测及其在溺死鉴定中的研究进展 被引量:6

Advances in the studies on detection of planktons by PCR and its application to diagnosis of drowning
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摘要 溺死鉴定是法医学中的重点和难点之一,目前在相关法医学案件中,主要采用法医病理学尸检与传统硅藻检验等方法综合分析进行鉴定。传统硅藻检验存在灵敏性较低,易于污染等不足。采用PCR法检测尸体不同脏器组织中是否存在水中浮游生物的DNA标记,适用范围广,信息量丰富,灵敏性和特异性较高,有较好的法医学应用前景,有望成为鉴定溺死的新方法。本文对相关研究进展进行综述,为相关研究和实践参考。 The diagnosis of drowning is one of the most important tasks and difficult issues in forensic science. Nowdays, investigation of death from drowning largely depends upon comprehensive analysis of autopsy findings in combination with detection of planktons in organs by traditional method in forensic practice. The traditional method for detection of diatoms is susceptible to contamination with relative low sensitiveness. Detection of plankton DNA by PCR can provide with abundant information with high sensitiveness and specificity, which has a wide applicability to diagnosis of drowning. In the present article, related progresses are reviewed as references to investigation of cases suspicious of drowning.
出处 《中国法医学杂志》 CSCD 2012年第3期209-211,共3页 Chinese Journal of Forensic Medicine
基金 公安部重点研究计划项目(20102DYJGDST017)
关键词 法医病理学 溺死 浮游生物 PCR forensic pathology death by drowning microorganism PCR
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参考文献20

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二级参考文献17

  • 1马书玲,李凡,秦豪杰,翟仙敦,莫耀南.40例可疑溺死案件脏器中硅藻检测的法医学分析[J].河南科技大学学报(医学版),2004,22(2):116-117. 被引量:5
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