摘要
目的 探讨肿瘤坏死因子 α(tumornecrosisfactoralpha ,TNF α)对周围神经损伤后感觉神经元的保护作用。方法 取Wistar大鼠 2 0只 ,分成TNF α和SAL两组。右侧坐骨神经切断后 ,将神经近断端套入硅胶管内。TNF α组自硅胶管内注入 16μlTNF α( 3 0U/ml)。SAL组在硅胶管内注射等量生理盐水。术后 2周观察脊神经节内感觉神经元乙酰胆碱脂酶 (AChE)、一氧化氮合酶 (NOS)的变化 ;测量脊神经节神经元的存活百分率及乙酰胆碱酯酶的平均灰度。结果 两组的乙酰胆碱脂酶、一氧化氮合酶均较正常神经元有显著性的变化 ,两组间神经元的存活百分率及乙酰胆碱酯酶的平均灰度的差异也存在非常显著性意义 (P <0 .0 1)。结论 TNF α对周围神经损伤后脊神经节感觉神经元有保护作用。
Objective To investigate the protective effect of tumor necrosis factor alpha(TNF α) on sensory neurons after nerve injury. Methods 20 Wistar rats were divided into 2 groups:TNF α and SAL. The right sciatic nerve was transected. After silicone tubulization of sciatic nerve, 16 μl TNF α (30 U/ml) and 16 μl 0 9 % normal saline were injected into silicone chamber of the TNF α and SAL group respectively. Two weeks postoperatively, bioactivity of AChE and NOS, survival rate, and AChE grey scale of the sensory neurons in DRG of the two groups were detected and compared. Results Compared with the control side, the activity of the two enzymes of both groups changed significantly. The survival rate of sensory neurons and the grey scale of AChE in TNF α group were significantly higher than those in SAL group ( P <0 01).Conclusions It was suggested that TNF alpha might protect sensory neurons after nerve injury.
出处
《中华手外科杂志》
CSCD
2000年第2期120-122,共3页
Chinese Journal of Hand Surgery
关键词
周围神经
创伤
损伤
肿瘤坏死因子
神经元
Peripheral nerves
Wounds and injuries
Tumor necrosis factor
Neurons,Afferent
