摘要
研究了不同初始酒精浓度对醋酸发酵的影响及分批补料对贝壳钙源发酵的影响。醋酸菌在初始酒精浓度为6%vol时的产酸速率、菌体生长速率都较快,且其发酵周期适中。在此基础上,研究了分批补料发酵过程中菌体生长、产物及副产物的合成规律。结果表明:分批补料发酵通过改善发酵的环境条件,进而提高钙离子的转化率。与分批发酵相比,发酵中钙离子的转化率由18.08%提高到了37.33%,钙离子的总浓度由16.96mg/mL提高到了33.99mg/mL。因此,分批补料发酵可显著提高代谢产物的产量,促进贝壳钙源的生物转化率。
The effects of initial alcohol concentrations on acetic acid fermentation and the influence of fed-batch on calcium biotransformation of mus-sel shell were investigated. When the initial alcohol concentration was 6%vol, the acetic acid production and growth rate of f Acetobacter sp. were faster, and the fermentation time was proper. Base on this study, the cell growth rate and synthetic rates of metabolites in fed-batch fermentation were investigated. The results showed that fed-batch fermentation can improve the calcium conversion rate through changing the fermentation environ-ment. Comparing with batch fermentation, the calcium conversion rate increased from 18.08% to 37.33%, and the total concentration of calcium in-creased from 16.96mg/ml to 33.99mg/ml in fed-bath fermentation. The fed-batch fermentation could significantly enhance the yield of acetic acid and promote the efficiency of calcium conversion.
出处
《中国酿造》
CAS
2012年第6期106-110,共5页
China Brewing
基金
浙江省优先主题资助项目(2008C13063)