摘要
[目的 ]探讨香烟对生殖作用的影响。 [方法 ]腹腔注射香烟烟雾水溶物后观察其对小鼠怀孕率、胚胎发育的影响 ,采用卵母细胞的体外培养和体外受精的方法 ,观察其对超排卵数、卵母细胞的成熟和受精能力影响。 [结果 ]香烟烟雾水溶物可以极显著地降低小鼠的妊娠率 ,抑制胚胎发育 ,还可以显著性地抑制卵母细胞的体内成熟 ,降低第一极体的释放率和卵母细胞的体外受精率 ,使卵母细胞在体外的生存能力明显降低。本实验结果还显示 ,香烟烟雾水溶物对小鼠生殖作用的损害和对卵母细胞成熟的抑制作用是可逆的 ,停止腹腔注射香烟烟雾水溶物和将卵母细胞或受精卵培养在正常的培养液中 ,小鼠的生育能力又能恢复、卵母细胞的第一极体的释放率以及体外受精率都明显提高。[结论 ]香烟烟雾水溶物可以破坏卵母细胞的成熟分裂 ,降低小鼠的生殖能力和生育能力 ,但这种毒性作用是可以逆转的 。
Objective]To study the effects of cigarette smoke on fertility of mouse [Methods]The pregnancy rates,the embryo development,the number of superovulation oocytes,the meiotic maturation and fertility of mouse oocytes were studied after intraperitoneal injection with water-soluble contents of cigarette smoke (WSCCS) using methods of cell culture and fertilization(IVF) of mouse oocyte in vitro [Results]WSCCS could significantly reduce the pregnancy rates,inhibit the embryo development,significantly inhibit the meiotic maturation and the extruding rates of the first polar body and fertilizing rates of mouse oocytes in vitro,as well asdepress the viability of oocytes in vitro The results also showed that the deleterious effects of WSCCS on mouse fertility and the meiotic maturation of mouse oocytes might be reversible,After stopping intruperitoneal injection with WSCCS and inoculating oocytes and zygotes in normal culture,the fertility of mouse and the extruding rates of the first polar body and INF rates obviously increased [Conclusion]WSCCS might destroy the oocytes meiosis,reduce the fertility and reproduction ability of female mouse But this toxic effects might disappear and the mouse fertility might be recoverable after stopping smoking
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2000年第3期145-158,共14页
Journal of Environment and Health
基金
江苏省自然科学基金!资助项目 (BK990 64)
关键词
香烟
烟雾
卵母细胞
水溶物
生殖影响
Cigarette smoke
Reproduction
Oocytes
Meiosis
External fertilization
Animal test
