摘要
通过腹腔注射链脲佐菌素(STZ)建立小鼠糖尿病模型后,运用体外受精技术研究母体高血糖对早期胚胎发育的影响。选取6～8周龄ICR母鼠2次腹腔注射小剂量STZ构建小鼠糖尿病模型,并设实验对照组。成功造模后对实验小鼠眼底静脉丛采血并测定14项血生化指标。通过孕马血清促性腺激素(PMSG)配合人绒毛膜促性腺激素(hCG)对实验母鼠进行超数排卵,所得卵子与正常ICR公鼠的精子体外受精并培养24h后,观察胚胎的发育情况并进行比较。结果表明实验组小鼠的空腹血糖明显升高(P<0.001),空腹血糖值≥9.5mmol/L的小鼠比例为76.6%。14项血生化指标中,实验组的谷丙转氨酶、肌酐、低密度脂蛋白、胆碱酯酶和糖化血红蛋白均较对照组显著升高(P<0.01)。高血糖模型小鼠卵子与正常精子体外受精后的受精率较正常小鼠低,且有血糖越高比例越低的趋势,说明体外受精技术虽可以改善糖尿病母鼠胚胎的受精率,但母体高血糖仍影响早期胚胎的发育。
To investigate the effects of maternal hypergalycemia on the development of early mouse embryo by the technique of in vitro fertilization, 6-8 week old ICR female mice models of diabetes mellitus （DM） were established by two intraperitoneal injections of Streptozotocin（STZ） , and the control group was set up. Blood of the mice were collected by posterior orbital venous plexus for the test of 14 biochemical indexes. The DM mice were superovulated by the intraperitoneal injection of PMSG and hCG, and oocytes were fertilized with sperms of normal male mice in vitro, and cultivated in HTF medium for 24 hours. The results showed that blood glucose levels of DM mice increased, significantly（ P〈0. 001）, blood glucose levels in 76. 6% of treated mice were above 9. 5 mmol/L. Compared with the control mice, Alanine aminotransferase, creatinine, low density lipoprotein, cholinesterase and glycated hemoglobin in DM mice were significantly increased（ P〈0. 01）. The fertilization rate of the oocytes of DM female mice with the sperms of normal male mice in HTF medium was lower than these of normal female mice. There was a tendency of a higher of blood glucose level with a lower of fertilization rate. We have a conclusion that hyperglycemia in maternal mice still has some adverse effects on early embryo development although in vitro fertilization technology can improve the rate of fertilization.
出处
《上海交通大学学报（农业科学版）》
2012年第3期31-35,共5页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市科委"创新行动计划"重点项目(10140900700)
关键词
链脲佐菌素
糖尿病模型
体外受精
受精率
streptozotocin
diabetes mellitus models
in vitro fertilization
ratio of fertilization
