摘要
工学校目的筛查特发性肺纤维化（IPF）患者支气管肺泡灌洗液（BALF）和血清中的差异蛋白，以期发现对诊断有价值的生物标志物。方法应用表面加强激光解吸一电离飞行时间质谱（SELDI—TOF—MS）技术对34例IPF患者（IPF组）和25例非吸烟健康者（对照组）血清和BALF进行检测获得蛋白质指纹图谱，用生物标志物模式软件、生物标志物导向软件对测得的数据进行处理。结果BALF中共检测出247个蛋白峰，与对照组比较，IPF组13个蛋白质峰差异有统计学意义，其中7个标志物峰强度显著增加，质荷比分别为2240．57、3459．32、3501．78、4146．50、4516．51、4615．88、5651．26（均P〈0．01）；6个标志物峰强度显著下降，质荷比分别为4989．91、5043．68、6968．76、11687．70、11782．10、14733．30（均P〈0．01）。血清中共检测出142个蛋白峰，与对照组比较，IPF组9个蛋白质峰差异有统计学意义，且9个标志物峰强度均显著增加，质荷比分别为3382．59、3453．39、4608．28、5825．48、8936．76、9164．27、11525．30、11689．40、11886．00（P〈0．05或P〈0．01）。结论SELDI—TOF—MS技术用于IPF患者BALF和血清蛋白质谱分析可筛选出有意义的差异表达蛋白，为进一步发现对IPF诊断有价值的生物标志物提供了实验基础。
Objective To screen potential proteins in bronchoalveolar lavage fluid （BALF） and serum samples obtained from patients with idiopathic pulmonary fibrosis （IPF）, for the purpose of discovering candidate biomarkers. Methods BALF and serum samples from 34 patients diagnosed IPF （IPF group） and 25 non-smoker heahhy controls （control group） were collected. Surface enhanced laser desorption/ionization time of flight mass spectrometry （SELDI-TOF-MS） was used to obtain protein fingerprints from BALF and serum samples. Biomarker Wizard and Biomarker Pattern were introduced in bioinformatics analysis. Results A total of 247 protein peaks were detected in BALF, and 13 peaks with statistical difference compared with control group. Among 13 detected protein peaks, 7 with mass/charge ratio （M/Z） values of 2240.57, 3459.32, 3501.78, 4146.50, 4516.51, 4615.88, 5651.26 were statistically up-regulated （all P〈0.01 ）; and 6 peaks with M/Z values of 4989.91, 5043.68, 6968.76, 11 687.70, 11 782.10, 14 733.30 were significantly down-regulated （all P〈0.01 ）. In addition, 142 protein peaks were differentially expressed in serum samples, and 9 peaks with statistical differences. Among them, 9 peaks with M/Z values of 3382.59, 3453.39, 4608.28, 5825.48, 8936.76, 9164.27, 11 525.30, 11 689.40 and 11 886.00 were significantly up-regnlated （P〈0.05 or P〈0.01 ）, compared with control group. Conclusions The present work demonstrated that SELDI-TOF-MS technology be capable of detecting differentially expressed protein peaks in BALF and serum samples from IPF patients. Further investigations were guaranteed to elucidate their potential diagnostic value in clinical practice.
出处
《中国危重病急救医学》
CAS
CSCD
北大核心
2012年第7期427-430,共4页
Chinese Critical Care Medicine
基金
天津市应用技术研究与前沿技术研究计划项目（11JCYBJC11800）
武警医学院博士启动基金（WBS200808）
