摘要
针对转基因大豆中普遍含有的35S启动子进行引物设计,以双链DNA染料SYBR GreenⅠ为荧光标记物,利用实时荧光定量PCR方法对大豆样品进行检测。该法检测转基因大豆的检测低限为0.005 nmol/L的35S启动子,线性范围达3个数量级,可快速区分转基因大豆和非转基因大豆,具有快速、简便、灵敏、安全、高通量、低成本等优点,可推广用于转基因植物产品的快速定量检测。
A real-time fluorescence quantitative PCR method was developed to detect genetically modified （ GM ） soybean using SYBR Green I,a double-stranded DNA-seleetive fluorescent dye. Special primers were used to amplify 35S promoter that was often used in GM soybeans. The fluorescence of SYBR Green I was used to monitor the quantity of PCR product. The results show that the detection limit for 35S promoter is 0. 005 nmol/L and the linear range is more than three orders of magnitude. The GM soybean and the non-GM soybean can be clearly discriminated. Thus, the method may become a convenient tool for daily GM food detection due to its rapidness, simplicity, sensitivity, safety, high throughput and low cost.
出处
《激光生物学报》
CAS
CSCD
2012年第3期279-282,共4页
Acta Laser Biology Sinica
基金
国家自然科学基金项目(No.81071790
30600128
6117707)
教育部科学技术研究重点项目(No.211131)
中国博士后科学基金(No.201003359)
广东省自然科学基金项目(No.7005825)
广东省优秀博士学位论文作者资助项目(No.SYBZZM201126)
广州市南沙区科技计划项目(No.RG201001003)
关键词
实时荧光定量PCR
转基因大豆
35S启动子
real-time fluorescence quantitative PCR
genetically modified soybean
35S promoter
