摘要
用分光光度法测定了茶多酚及其氧化产物对不同体系产生的活性氧自由基O2· - 和·OH的清除作用。结果表明 :对由光照核黄素体系产生的O2· - ,在试验质量浓度范围 ( 8 3～ 1 67ug mL)内茶多酚及其氧化产物的最大清除率分别为 96 6%和 92 1 %。对由 2 脱氧 D 核糖体系产生的·OH ,在实验质量浓度范围 ( 5～ 80 0ug mL)内茶多酚及其氧化产物的最大清除率分别为83 4%和 88 5%。茶多酚氧化产物 ,SOD粗酶液 ,茶多酚对邻苯三酚自氧化产生的O2· - 均表现出较强的抑制作用 ,抑制率分别为 66 5% ,47 6% ,45 3%。茶多酚及其氧化产物与SOD有显著的协同增效作用 ,但氧化产物的效果强于茶多酚。
The radical scavenging effects of tea polyphenol(TP) and its oxidant were investigated with spectrophotomeric method in different systems.The result showed that,both tea polyphenol and its oxidant had strong scavenging effects to both hydroxyl radical and superoxide radical generated by different systems.In the deoxyribose system,in the test concentration range from 83 μg/mL to 167 μg/mL,the maximum scavenging rate of TP and its oxidant to superoxide radical were 966% and 921% respectively.In the flavins system the maximum scavenging rate of TP and its oxidant to hydroxyl radical were 834% and 885% respectively in the test concentration range from 5 μg/mL to 800 μg/mL.In the pyrogallol autoxidation system,the maximum inhibition rate of TP oxidant,crude SOD and TP were 665%,476% and 453% respectively,and both TP and its oxidant have building action to crude SOD,but the effect of TP oxidant was stronger than TP.
出处
《精细化工》
EI
CAS
CSCD
北大核心
2000年第4期241-244,共4页
Fine Chemicals
