摘要
目的探讨和评价hsp65and rpoB PCR-RFLP用于龟/脓肿分枝杆菌复合群种的快速鉴定。方法收集经PNB/TCH鉴别培养基表型鉴定和16s rRNA基因测序鉴定为龟/脓肿分枝杆菌复合群的临床分离菌株,用hsp65and rpoBPCR-RFLP进行种/亚种鉴定。结果经表型鉴定为非结核分枝杆菌的27株临床菌株,16s rRNA基因测序分析与龟/脓肿分枝杆菌的同源性达到99.7%。经hsp65PCR-RFLP and rpoBPCR-RFLP鉴定18株为脓肿分枝杆菌(M.abscessus),4株为溃疡分枝杆菌(M.absecces),另5株表现为独特的指纹特征,可能是一个新的亚种。结论能够快速进行龟/脓肿分枝杆菌复合群种/亚种的鉴定。
The Mycobacteriurn chelonae/abscessus （M. chelonae/abscessus） complex belongs to the rapidly growing ge- nus Mycobacterium （RGM）. It is one of the most important pathogenic members of Mycobacteriurn leading to nosocomial infec- tions and outbreaks. It includes members of M. chelonae, M. irnrnunogenum, M. abscessus, M. massiliense, and M. bolletii. In order to investigate the epidemiological characteristics of the M. chelonae/abscessus complex in China and to conduct the mo lecular methods for species identification of M. chelonae/abscessus, we collected clinical M. chelonae/abscessus complex strains identified by phenotypic tests. Members were verified by sequencing of 16S rRNA. Species and subspecies were identified by hsp65 and rpoB PCR-RFLP methods. In total, 27 clinical specimens were identified as Mycobacteriurn chelonae/abscessus com- plex by phenotypic tests. 16s rRNA gene sequence analysis of all 27 clinical samples shared over 99. 7~/oo similarity with M. chelonae and M. abscessus. Species identification with hsp65 PCR-RFLP and rpoB PCR RFLP revealed that 18 specimens were M. abscessus and 4 were M. absecces. The remaining 5 samples displayed a pattern that failed to match any previously reported pattern. Thus, this might represent a novel species that is part of the Mycobacteriurn chelonae/abscessus complex. We identified that a majority of the chronic lung infection in China is caused by the M. chelonae/abscessus complex. Specifically, the M. abscessus species might be the most infectious, while other species in the complex can still cause infection. Interesting- ly, there may be a novel or previously unidentified species that is a part of the complex. Finally, we show that species identifi- cation can be carried out more accurately by combined use of hsp65 and rpoB PCR-RFLP.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2012年第7期645-652,共8页
Chinese Journal of Zoonoses
基金
supported by the projects 2008ZX100/03-010 and 2011ZX10004-001 of China Mega-Project for Infectious Disease~~
