摘要
为探究Ghrelin免疫阳性细胞山羊胃肠道的定位分布、形态特征与发育性变化,选用0、30、60、90、120、150和180日龄雌性济宁青山羊,按试验要求取材后进行免疫组织化学方法染色。结果显示,Ghrelin免疫阳性细胞主要分布在黏膜上皮、腺体和固有层,有"闭合型"和"开放型"2种类型。2种类型细胞在皱胃、十二指肠、空肠、回肠、盲肠和结肠均有分布,但未在瘤胃、网胃、瓣胃和直肠中检出。Ghrelin阳性细胞在皱胃中最多,由皱胃至结肠逐渐减少,"闭合型"细胞所占比例也由皱胃至结肠逐步减少。从0～120日龄,皱胃和空肠中阳性细胞数随着年龄增长而增加,150和180日龄有所下降;十二指肠中阳性细胞在90日龄达到高峰。回肠、盲肠和结肠中阳性细胞数较少,均呈现随日龄增长而缓慢增长的趋势。Ghrelin免疫阳性细胞的数量变化表明Ghrelin可能与山羊胃肠道发育密切相关。
In the present study,the distribution,morphological characteristics and developmental changes of Ghrelin-immunopositive（Ghrelin-ip） cells in the gastrointestinal tract of goat were investigated.Using the method of immunohistochemistry,Ghrelin-ip cells were showed in the gastrointestinal tract of Jining Gray goat at the ages of day 0,30,60,90,120,150 and 180.Ghrelin-ip cells classified into open-and closed-type cells were found to be localized in the mucosa epithelium,glands and lamina propria of abomasums,duodenum,jejunum,ileum,cecum and colon,but not in rumen,reticulum,omasum and rectum.The greatest number of Ghrelin-ip cells was found in the abomasums and the Ghrelin-ip cell density gradually decreased from the abomasums to the colon.The percentage of closed-type cells to all immunopositive cells also decreased from the abomasums to the colon.With the goat growth,the densities of Ghrelin-ip cells in the abomasums and jejunum increased from ages of day 0 to 120,but slightly decreased at ages of day 150 and 180.There was also a steady increase in the number of Ghrelin-ip cells from postnatal day 0 to 90 in duodenum,which reached a peak and remained steady.Very few Ghrelin-ip cells were found in the ileum,cecum and colon,and the densities increased with the age growth.The change of Ghrelin-immunopositive cells in the gastrointestinal tract of growing goats suggests that Ghrelin may be involved in gastrointestinal tract development.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2012年第7期1042-1048,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金(30871832)
山东省自然科学基金(ZR2009DM027)
关键词
GHRELIN
济宁青山羊
胃肠道
免疫组化
Ghrelin； Jining Gray goat； gastrointestinal tract； immunohistochemistry
