摘要
本研究测定了NIDDM病人(n=6)和正常人(n=7)循环血中单核细胞(Mo)结合^(125)I-胰岛素总能力、胰岛素-受体复合物进入能力、胰岛素降解能力和趋溶酶体制剂氯喹对受体后过程的影响。结果显示NIDDM时Mo对胰岛素的结合能力、进入能力和降解能力均明显低于正常人。这些改变可能与NIDDM胰岛素抵抗性有关。
We studied the total cell-associated A14-125I-insulin radioactivity (TBd, including surface-bound and internalized radioactivity), insulin internalization (In), and the effects of lysosomotropic agent Chloroquine (CQ) on postreceptor processing in peripheral monocytes (Mo) from the patients with non-insulin-dependent diabetes mellitus (NIDDM, n=6) and normal controls (n= 7). The results indicate that 1) insulin is able to bind to and internalize into Mo; 2) the abilities of TBd and In of Mo from the patients with NIDDM are significantly decreased (4.03±0.40 vs 9.40±0.87 and 1.95±0.44 vs 6.06±0.63 pg/107Mo); 3) intracellular insulin degradation is reduced in Mo from the diabetics; and 4) lysosomotropic agent CQ, has no effects on intracellular A14-125I-insulin radioactivity in Mo from both diabetic and normal subjects. These defects may be related to the cellular insulin resistance present in patients with NIDDM.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
1990年第3期131-133,共3页
Chinese Journal of Endocrinology and Metabolism
关键词
糖尿病
胰岛素
单核细胞
受体
Non-insulin-dependent diabetes mellitus Receptor
