摘要
利用广西某温泉水样分离At.f菌并进行纯化,采用超声波诱变研究对鄂西鲕状赤铁矿磁选精矿脱磷技术。结果表明:1)超声波诱变可以提高At.f菌的活性,当超声波频率为59kHz、诱变时间为20min时,体系中Fe2+完全氧化所需时间为20min;2)超声波诱变20min后的At.f菌接种比为15%~20%时,30d铁矿的脱磷效果为85%以上;3)培养基中ρ(Fe2+)≥9g/L时,诱变后的菌株对于鲕状赤铁矿磁选精矿的脱磷率为80%以上。
Strains of Acidithiobacillus ferrooxidans (At. f) were separated and purified from water samples collected from a hot spring in Guangxi province. The purified At. f was used in the leaching of phosphorous in magnetic concentrate of oolitic hematite in the west of Hubei province. The results indicate that: 1) The optimum ultrasonic rnutagenesis (59 kHz) time, to improve oxidation activity with At. f, was showed at 20 min. In this condition, the needed time of Fe2+ complete oxidization was 20 rnin in the system; 2) The rate of phosphorus, which was leached by ultrasonic mutagenesis(59 kHz, 20 min) purified At. f ,with the range of inoculum ratio between 15% and 20%, was more than 85%; 3) With ferrous iron concentration the optimal leaching of phosphorus, from the magnetic concentrate of oolitic hematite in the west of Hubei province, the best result was more than 80% obtained with the bacteria when ρ(Fe2+)≥9g/L.
出处
《武汉理工大学学报》
CAS
CSCD
北大核心
2012年第6期82-86,共5页
Journal of Wuhan University of Technology
基金
国家科技部国际科技合作项目(2011DFR00110)
国家"十一五"支撑计划(2007BAB15B01)
贵州省科技厅科技基金(黔科合J字[2009]2105号)
贵州省自然科学基金(黔教科20090076)
关键词
超声波
诱变
嗜酸氧化亚铁硫杆菌
鲕状赤铁矿
脱除磷
ultrasonic
rnutagenesis
Acidithiobacillus ferrooxidans (At. f)
oolitic hematite
phosphorus leaching