摘要
参考果蝇与蚤状溞序列进行了日本绒螯蟹放流群体的线粒体ＤＮＡ １２Ｓ ｒＲＮＡ基因片段的引物设计、ＰＣＲ扩增及序列测定，得到４５７ｂｐ的碱基序列，其Ａ、Ｔ、Ｇ、Ｃ含量分别为１５８ｂｐ（３４．５７％）、１７８ｂｐ（３８．９５％）、５１ｂｐ（１１．１６％）、７０ｂｐ（１５．３２％），与果蝇与蚤状溞相同基因片段序列含量相似。
The primers of mitochondrial DNA 12S rRNA region of stocked population of Japanese mitten crab were designed by using the sequences of Drosophila yakuba and Daphnia pulex. The 457 base pairs that encode the 12S rRNA region were amplified and sequenced. The A, T, G, C contents of the sequences were 158bp(34.57%), 178bp (38.95% ), 51bp(11. 16% ), and 70bp(15.32% ) respectively, which are similar with the same region of Drosophila yakuba and Daphnia pulex.
出处
《海洋湖沼通报》
CSCD
北大核心
2000年第1期47-51,共5页
Transactions of Oceanology and Limnology
基金
国家自然科学基金!(编号:39970578)
关键词
日本绒螯蟹
放流群体
线粒体
DNA
Eriocheir japonica stocked population mitochondrial DNA 12S rRNA sequences
