摘要
用免疫组织化学法研究肝细胞癌 (HCC)及癌旁肝组织血管内皮生长因子 (VEGF)的表达与微血管 (MV)密度的关系。VEGF在癌旁肝组织中的阳性率较HCC中者为高 (85 .4 %vs.66 .7% ;P <0 .0 5) ;其表达强度在两者中差异无显著性 (P >0 .0 5) ;其阳性信号主要位于癌细胞、癌旁肝细胞和血管内皮细胞胞浆。HCC中MV密度明显高于癌旁肝组织 (P <0 .0 1 ) ,并随癌细胞分化程度的降低而升高。结果提示 ,在HCC发生发展过程中VEGF并非唯一刺激MV生成的因子 ;
The relationship between vascular endothelial growth factor (VEGF) expression and microvessel density was studied with immunohistochemical method in hepatocellular carcinoma (HCC) and pericarcinomatous liver tissue. The positive rate of VEGF in HCCs was significantly lower than in surrounding liver tissues (66.7% vs. 85.4%, P<0.05). There was no significant difference between HCC and pericarcinomatous liver tissue on expressive intensity of VEGF. The positive signal of VEGF was mainly localized in cytoplasma of cancer cells, pericarcinomatous hepatocytes, and vascular endothelial cells. The microvessel density in HCC was higher than in pericarcinomatous liver tissue and closely correlated to differentiated degree of cancer cells (r s=0.5870; r s=0.8235). The poorer cancer cell differentiation, the higher microvessel density. The results suggest that VEGF may not be the sole factor that stimulates angiogenesis in HCC genesis and development. To detect microvessel density in judging prognosis and biological behavior of HCC is more important than that of VEGF. [
出处
《湖南医科大学学报》
CSCD
2000年第2期132-134,共3页
Bulletin of Hunan Medical University
关键词
肝细胞癌
癌旁肝组织
微血管密度
VEGF
liver neoplasms
carcinomas
blood vessels
endothelial growth factors
immunohis-tochemistry
